Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.28 extracted from

  • Yu, C.K.; Wang, C.J.; Chew, Y.; Wang, P.C.; Yin, H.S.; Kao, M.C.
    Functional characterization of Helicobacter pylori 26695 sedoheptulose 7-phosphate isomerase encoded by hp0857 and its association with lipopolysaccharide biosynthesis and adhesion (2016), Biochem. Biophys. Res. Commun., 477, 794-800 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
gel filtration Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori O25528
-
-
Helicobacter pylori 26695 O25528
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-sedoheptulose 7-phosphate
-
Helicobacter pylori D-glycero-D-manno-heptose 7-phosphate
-
?
D-sedoheptulose 7-phosphate
-
Helicobacter pylori 26695 D-glycero-D-manno-heptose 7-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 24000, SDS-PAGE, 2 * 23100, calculated from sequence, 2 * 23200, mass spectrometry Helicobacter pylori

Synonyms

Synonyms Comment Organism
GmhA
-
Helicobacter pylori
HP0857
-
Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
33.1
-
D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori

General Information

General Information Comment Organism
physiological function a knockout mutant exhibits a decreased growth rate and displays a deep rough type of lipopolysaccharide structure. It has a slight decrease in its motility and is more susceptible to novobiocin and detergents Triton X-100 and SDS. The adhesive capacity of the mutant to AGS cells is reduced significantly, and most of the infected cells don't show a classic hummingbird phenotype Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
71.9
-
D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori