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Literature summary for 5.3.1.27 extracted from

  • Rozova, O.N.; But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Mustakhimov, I.I.; Trotsenko, Y.A.
    Characterization of two recombinant 3-hexulose-6-phosphate synthases from the halotolerant obligate methanotroph Methylomicrobium alcaliphilum 20Z (2017), Biochemistry (Moscow), 82, 176-185 .
    View publication on PubMed
Search for more literature for 5.3.1.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 PAGE Methylotuvimicrobium alcaliphilum
70000
-
 PAGE Methylotuvimicrobium alcaliphilum

Organism

Organism UniProt Comment Textmining
Methylotuvimicrobium alcaliphilum G4T0K4
-
-
Methylotuvimicrobium alcaliphilum G4T2U5 hexulose-6-phosphate synthase, activity of EC 4.1.2.43, and isomerase
-
Methylotuvimicrobium alcaliphilum 20Z G4T0K4
-
-
Methylotuvimicrobium alcaliphilum 20Z G4T2U5 hexulose-6-phosphate synthase, activity of EC 4.1.2.43, and isomerase
-

Subunits

Subunits Comment Organism
dimer 2 * 20000, SDS-PAGE, 2 * 19200, calculated from sequence Methylotuvimicrobium alcaliphilum
dimer 2 * 40000, SDS-PAGE, 2 * 41900, calculated from sequence Methylotuvimicrobium alcaliphilum

Synonyms

Synonyms Comment Organism
Hpi1
-
 Methylotuvimicrobium alcaliphilum
Hpsi2
-
 Methylotuvimicrobium alcaliphilum
PHI
-
 Methylotuvimicrobium alcaliphilum

General Information

General Information Comment Organism
physiological function 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexulose isomerase (PHI) are encoded by the genes Hps and Phi and the fused gene Hps-Phi (Hpsi2). Both 3-hexulose-6-phosphate synthase plus 6-phospho-3-hexulose isomerase and the fused gene product catalyze formation of fructose 6-phosphate from formaldehyde and ribulose 5-phosphate with activities of 172 and 22 U/mg, respectively. the fusion protein Hpsi2 does not show isomerase activity, but the sequences corresponding to its HPS and PHI regions, when expressed separately, produce active enzymes Methylotuvimicrobium alcaliphilum
physiological function 3-hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexulose isomerase (PHI) are encoded by the genes Hps and Phi and the fused gene Hps-Phi. Both 3-hexulose-6-phosphate synthase plus 6-phospho-3-hexulose isomerase and the fused gene product catalyze formation of fructose 6-phosphate from formaldehyde and ribulose 5-phosphate with activities of 172 and 22 U/mg, respectively Methylotuvimicrobium alcaliphilum