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Literature summary for 5.3.1.26 extracted from

  • Park, H.Y.; Park, C.S.; Kim, H.J.; Oh, D.K.
    Substrate specificity of a galactose 6-phosphate isomerase from Lactococcus lactis that produces d-allose from d-psicose (2007), J. Biotechnol., 132, 88-95.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
58
-
D-allose
-
Lactococcus lactis
143
-
D-altrose
-
Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect of Na+, K+, Mn2+, Zn2+, Ba2+, Cu2+, Co2+, Ca2+, Mg2+, Fe3+, Al3+ Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
LacA subunit, SDS-PAGE Lactococcus lactis
19000
-
LacB subunit, SDS-PAGE Lactococcus lactis
135500
-
native enzyme by gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P23495
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant protein by HiTrap Q HP column chromatography Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.79
-
of the purified enzyme to produce D-allose Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 D-lyxose
-
Lactococcus lactis D-xylulose + D-xylose
-
r
2 D-psicose
-
Lactococcus lactis D-allose + D-altrose
-
r
2 L-lyxose
-
Lactococcus lactis L-xylulose + L-xylose
-
r
D-allose
-
Lactococcus lactis D-psicose + D-altrose
-
r
D-altrose
-
Lactococcus lactis D-psicose + D-allose
-
r
D-arabinose
-
Lactococcus lactis D-ribulose + D-ribose
-
r
D-fructose
-
Lactococcus lactis D-glucose + D-mannose
-
r
D-galactose
-
Lactococcus lactis D-tagatose + D-talose
-
r
D-Galactose 6-phosphate
-
Lactococcus lactis D-Tagatose 6-phosphate
-
r
D-glucose
-
Lactococcus lactis D-fructose + D-mannose
-
r
D-glucose
-
Lactococcus lactis D-sorbose + D-idose
-
r
D-mannose
-
Lactococcus lactis D-fructose + D-glucose
-
r
D-ribose
-
Lactococcus lactis D-ribulose + D-arabinose
-
r
D-ribulose
-
Lactococcus lactis D-ribose + D-arabinose
-
r
D-sorbose
-
Lactococcus lactis D-glucose + D-idose
-
r
D-talose
-
Lactococcus lactis D-tagatose
-
r
D-Xylose
-
Lactococcus lactis D-Xylulose
-
r
D-xylulose
-
Lactococcus lactis D-xylose + D-lyxose
-
r
L-fructose
-
Lactococcus lactis L-glucose + L-mannose
-
r
L-galactose
-
Lactococcus lactis L-tagatose + L-talose
-
r
L-glucose
-
Lactococcus lactis L-fructose
-
r
L-ribulose
-
Lactococcus lactis L-ribose + L-arabinose
-
r
L-tagatose
-
Lactococcus lactis L-galactose + L-talose
-
r
L-Xylose
-
Lactococcus lactis L-Xylulose
-
r
L-xylulose
-
Lactococcus lactis L-xylose + L-lyxose
-
r

Subunits

Subunits Comment Organism
octamer hetero-octamer, LacA and LacB subunits Lactococcus lactis

Synonyms

Synonyms Comment Organism
galactose-6-phosphate isomerase
-
Lactococcus lactis
LacAB
-
Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Lactococcus lactis

pH Stability

pH Stability pH Stability Maximum Comment Organism
9
-
-
Lactococcus lactis