Cloned (Comment) | Organism |
---|---|
into the vector pGEM-T Easy, and subsequently into the vector pET15b for expression in Escherichia coli ER2566 cells | Caldicellulosiruptor saccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
141 | - |
L-fucose | - |
Caldicellulosiruptor saccharolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
determined by SDS-PAGE | Caldicellulosiruptor saccharolyticus |
68110 | - |
theoretical | Caldicellulosiruptor saccharolyticus |
204000 | - |
homotrimer, determined by gel filtration | Caldicellulosiruptor saccharolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Fucose | Caldicellulosiruptor saccharolyticus | - |
L-Fuculose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor saccharolyticus | A4XJ56 | - |
- |
Purification (Comment) | Organism |
---|---|
on a His-Trap HP column | Caldicellulosiruptor saccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
purification step crude extract, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
10 | - |
purification step heat treatment, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
76 | - |
purification step His-Trap column, substrate L-fucose | Caldicellulosiruptor saccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Fucose | - |
Caldicellulosiruptor saccharolyticus | L-Fuculose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 68000 Da | Caldicellulosiruptor saccharolyticus |
Synonyms | Comment | Organism |
---|---|---|
L-Fucose isomerase | D-arabinose isomerase, bifunctional enzyme | Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
L-fucose isomerization | Caldicellulosiruptor saccharolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1985 | - |
L-fucose | - |
Caldicellulosiruptor saccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
L-fucose isomerization | Caldicellulosiruptor saccharolyticus |