Cloned (Comment) | Organism |
---|---|
into the vector pET-15b | Streptomyces coelicolor |
Crystallization (Comment) | Organism |
---|---|
the structure of the PriA mutant R139N is determined to a resolution of 1.95 A | Streptomyces coelicolor |
Protein Variants | Comment | Organism |
---|---|---|
R139N | mutant, used for crystallization, steady-state Michaelis-Menten enzyme kinetic is studied | Streptomyces coelicolor |
R19A | mutant, steady-state Michaelis-Menten enzyme kinetic is studied | Streptomyces coelicolor |
S81T | mutant, steady-state Michaelis-Menten enzyme kinetic is studied | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA, PRA isomerase activity | Streptomyces coelicolor | |
0.005 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA, PRA isomerase activity | Streptomyces coelicolor | |
0.0083 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA mutant R19A, PRA isomerase activity | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | Streptomyces coelicolor | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | P16250 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Streptomyces coelicolor | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosyl anthranilate isomerase | - |
Streptomyces coelicolor |
phosphoribosyl isomerase A | - |
Streptomyces coelicolor |
PRA isomerase | - |
Streptomyces coelicolor |
PriA | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
activity assay | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA mutant R19A, PRA isomerase activity | Streptomyces coelicolor | |
3.4 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA, PRA isomerase activity | Streptomyces coelicolor | |
12 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | PriA, PRA isomerase activity | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
metabolism | phosphoribosyl isomerase A takes part in histidine and tryptophan biosynthesis | Streptomyces coelicolor |