Literature summary for 5.3.1.24 extracted from

Henn-Sax, M.; Thoma, R.; Schmidt, S.; Hennig, M.; Kirschner, K.; Sterner, R.
Two (betaalpha)8-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates (2002), Biochemistry, 41, 12032-12042.

Search for more literature for 5.3.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of trpF mutants, expression in Escherichia coli BL21(DE3)	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Thermotoga maritima
X-ray structure of a complex between TrpF and its product analogue reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
C7A	catalytically inactive mutant	Thermotoga maritima
D126N	mutant with drastically reduced activity	Thermotoga maritima
D126N/C7A	catalytically inactive double mutant	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00028	-	N-(5-phospho-beta-D-ribosyl)anthranilate	pH 7.5, 25°C, dimer	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate	Thermotoga maritima	involved in tryptophan biosynthesis from chorismate	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q56320	monofunctional enzyme	-

Purification (Commentary)

Purification (Comment)	Organism
TrpF mutants C7A, D126N and C7A/D126N double mutant	Thermotoga maritima

Reaction

Reaction	Comment	Organism	Reaction ID
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	catalytic mechanism	Thermotoga maritima	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate	high catalytic efficiency	Thermotoga maritima	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	-	?
N-(5-phospho-beta-D-ribosyl)anthranilate	reaction mechanism involving general acid-base catalysis and a Schiff base intermediate, Cys-7 and Asp-126 are important for the reaction	Thermotoga maritima	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	-	?
N-(5-phospho-beta-D-ribosyl)anthranilate	involved in tryptophan biosynthesis from chorismate	Thermotoga maritima	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
TrpF	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	extremely thermostable	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.7	-	N-(5-phospho-beta-D-ribosyl)anthranilate	pH 7.5, 25°C, dimer	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Thermotoga maritima

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Release 2023.1 (January 2023)