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Literature summary for 5.3.1.23 extracted from
- A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme (2009), Mol. Cell. Proteomics, 8, 2308-2320.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| RhoA | MRDI is induced by RhoA and mediates RhoA-dependent cell invasion | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pCMV plasmid for expression in mammalian cells and into pMS356 plasmid for esxpression in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C168S | inactive mutant | Homo sapiens |
| D248A | inactive mutant | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| RNAi | stable knockdown of MRDI is carried out in A-375 cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
| plasma membrane | - |
Homo sapiens | 5886 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-5-thio-alpha-D-ribose 1-phosphate | Homo sapiens | - |
S-methyl-5-thio-D-ribulose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| A-375 cell | - |
Homo sapiens | - |
| FOM71 cell | - |
Homo sapiens | - |
| FOM78 cell | - |
Homo sapiens | - |
| melanocyte cell line | - |
Homo sapiens | - |
| melanoma cell line | - |
Homo sapiens | - |
| NHEM2493 cell | - |
Homo sapiens | - |
| NHEM693 cell | - |
Homo sapiens | - |
| WM-35 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-methyl-5-thio-alpha-D-ribose 1-phosphate | - |
Homo sapiens | S-methyl-5-thio-D-ribulose 1-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylthioribose-1-phosphate isomerase | - |
Homo sapiens |
| MRDI | mediator of Rho-dependent invasion | Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | RhoA induces expression of MRDI | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | MRDI is induced in metastatic cells by constitutive RhoA activation and promotes cell invasion | Homo sapiens |
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