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show all sequences of 5.3.1.20

Purification, crystallization, and properties of D-ribose isomerase from Mycobacterium smegmatis

Izumori, K.; Rees, A.W.; Elbein, A.D.; J. Biol. Chem. 250, 8085-8087 (1975)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Mycolicibacterium smegmatis
Inhibitors
Inhibitors
Commentary
Organism
Structure
L-arabitol
-
Mycolicibacterium smegmatis
ribitol
-
Mycolicibacterium smegmatis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
D-ribose
-
Mycolicibacterium smegmatis
5.3
-
L-Lyxose
-
Mycolicibacterium smegmatis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, Km: 0.0004 mM
Mycolicibacterium smegmatis
Mg2+
divalent cation required, Km: 0.018 mM
Mycolicibacterium smegmatis
Mn2+
divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM
Mycolicibacterium smegmatis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
174000
sedimentation equilibrium analysis
Mycolicibacterium smegmatis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycolicibacterium smegmatis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.84
-
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Allose
-
2645
Mycolicibacterium smegmatis
?
-
-
-
-
D-Lyxose
-
2645
Mycolicibacterium smegmatis
?
-
-
-
-
D-Ribose
-
2645
Mycolicibacterium smegmatis
D-Ribulose
-
2645
Mycolicibacterium smegmatis
-
L-Rhamnose
-
2645
Mycolicibacterium smegmatis
L-Rhamnulose
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 42000-44000, SDS-PAGE in presence of Mn2+
Mycolicibacterium smegmatis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Mycolicibacterium smegmatis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
pH 6.5: about 45% of maximal activity, pH 9.5: about 55% of maximal activity
Mycolicibacterium smegmatis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Mycolicibacterium smegmatis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-arabitol
-
Mycolicibacterium smegmatis
ribitol
-
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4
-
D-ribose
-
Mycolicibacterium smegmatis
5.3
-
L-Lyxose
-
Mycolicibacterium smegmatis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, Km: 0.0004 mM
Mycolicibacterium smegmatis
Mg2+
divalent cation required, Km: 0.018 mM
Mycolicibacterium smegmatis
Mn2+
divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM
Mycolicibacterium smegmatis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
174000
sedimentation equilibrium analysis
Mycolicibacterium smegmatis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Mycolicibacterium smegmatis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.84
-
-
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Allose
-
2645
Mycolicibacterium smegmatis
?
-
-
-
-
D-Lyxose
-
2645
Mycolicibacterium smegmatis
?
-
-
-
-
D-Ribose
-
2645
Mycolicibacterium smegmatis
D-Ribulose
-
2645
Mycolicibacterium smegmatis
-
L-Rhamnose
-
2645
Mycolicibacterium smegmatis
L-Rhamnulose
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 42000-44000, SDS-PAGE in presence of Mn2+
Mycolicibacterium smegmatis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Mycolicibacterium smegmatis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
9.5
pH 6.5: about 45% of maximal activity, pH 9.5: about 55% of maximal activity
Mycolicibacterium smegmatis
Other publictions for EC 5.3.1.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
2649
Shukla
-
Studies on sugar isomerases of ...
Lactobacillus sp.
J. Basic Microbiol.
25
273-277
1985
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1
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1
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1
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1
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1
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-
-
-
-
-
-
1
-
-
-
1
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-
-
-
-
-
-
-
-
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-
2648
Elbein
D-Ribose isomerase ...
Mycolicibacterium smegmatis
Methods Enzymol.
89
547-550
1982
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-
-
-
-
1
-
2
-
3
-
-
-
1
-
-
1
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-
-
-
1
4
1
-
-
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-
1
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
3
-
-
-
-
-
1
-
-
-
1
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2650
Izumori
-
Induction of D-ribose isomeras ...
Mycolicibacterium smegmatis
Agric. Biol. Chem.
44
223-225
1980
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-
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1
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2
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2
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-
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-
2629
Izumori
Evidence that the isomerizatio ...
Mycolicibacterium smegmatis
J. Bacteriol.
126
553-555
1976
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-
-
-
-
-
-
-
-
-
-
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2
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-
1
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2
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2
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1
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2
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-
2
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-
-
-
-
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2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
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-
-
-
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-
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1
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2
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3
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2
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1
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3
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2
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-
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-
-
-
-
-
-
-
-
-
2645
Izumori
Purification, crystallization, ...
Mycolicibacterium smegmatis
J. Biol. Chem.
250
8085-8087
1975
-
-
-
1
-
-
2
2
-
3
1
-
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
2
-
3
1
-
-
-
-
1
-
-
1
-
4
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-