BRENDA - Enzyme Database show
show all sequences of 5.3.1.15

Efficient biotransformation of D-fructose to D-mannose by a thermostable D-lyxose isomerase from Thermosediminibacter oceani

Yu, L.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.; Process Biochem. 51, 2026-2033 (2016)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%
Thermosediminibacter oceani
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
1 mM, 64% residual activity
Thermosediminibacter oceani
Cu2+
1 mM, 16% residual activity
Thermosediminibacter oceani
Zn2+
1 mM, 13% residual activity
Thermosediminibacter oceani
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15.5
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
27.3
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
32.8
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM, 9.1fold activation
Thermosediminibacter oceani
Fe2+
1 mM, 2.1fold activation
Thermosediminibacter oceani
Mn2+
1 mM, 12.5fold activation
Thermosediminibacter oceani
Ni2+
1 mM, 11.8fold activation
Thermosediminibacter oceani
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Thermosediminibacter oceani
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermosediminibacter oceani
D9RZ53
-
-
Thermosediminibacter oceani DSM 1664
D9RZ53
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani
D-mannose
-
-
-
r
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani DSM 16646
D-mannose
-
-
-
r
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani DSM 1664
D-mannose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani
D-Xylulose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani DSM 16646
D-Xylulose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani DSM 1664
D-Xylulose
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 22000, SDS-PAGE
Thermosediminibacter oceani
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
65
-
-
Thermosediminibacter oceani
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
half-life 5.64 h
Thermosediminibacter oceani
80
-
half-life 0.77 h
Thermosediminibacter oceani
85
-
half-life 0.2 h
Thermosediminibacter oceani
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
17.2
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
51.8
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
94.8
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermosediminibacter oceani
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
more than 50% of maximum activity
Thermosediminibacter oceani
Application (protein specific)
Application
Commentary
Organism
synthesis
under optimum conditions, 101.6 g/l D-mannose is produced from 400 g/l D-fructose after reaction for 9 h, giving a conversion yield of 25.4%
Thermosediminibacter oceani
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
1 mM, 64% residual activity
Thermosediminibacter oceani
Cu2+
1 mM, 16% residual activity
Thermosediminibacter oceani
Zn2+
1 mM, 13% residual activity
Thermosediminibacter oceani
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15.5
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
27.3
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
32.8
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
1 mM, 9.1fold activation
Thermosediminibacter oceani
Fe2+
1 mM, 2.1fold activation
Thermosediminibacter oceani
Mn2+
1 mM, 12.5fold activation
Thermosediminibacter oceani
Ni2+
1 mM, 11.8fold activation
Thermosediminibacter oceani
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
gel filtration
Thermosediminibacter oceani
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani
D-mannose
-
-
-
r
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani DSM 16646
D-mannose
-
-
-
r
D-fructose
reaction of EC 5.3.1.7
749201
Thermosediminibacter oceani DSM 1664
D-mannose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani
D-Xylulose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani DSM 16646
D-Xylulose
-
-
-
r
D-Lyxose
best substrate
749201
Thermosediminibacter oceani DSM 1664
D-Xylulose
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 22000, SDS-PAGE
Thermosediminibacter oceani
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
65
-
-
Thermosediminibacter oceani
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
half-life 5.64 h
Thermosediminibacter oceani
80
-
half-life 0.77 h
Thermosediminibacter oceani
85
-
half-life 0.2 h
Thermosediminibacter oceani
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
17.2
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
51.8
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
94.8
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Thermosediminibacter oceani
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
more than 50% of maximum activity
Thermosediminibacter oceani
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.63
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
2.88
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
3.35
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.63
-
D-fructose
pH 6.5, 65C
Thermosediminibacter oceani
2.88
-
D-mannose
pH 6.5, 65C
Thermosediminibacter oceani
3.35
-
D-Lyxose
pH 6.5, 65C
Thermosediminibacter oceani
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
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1
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6
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1
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747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
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-
-
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4
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1
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1
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1
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749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
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-
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3
3
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4
1
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2
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6
1
1
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3
3
1
1
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1
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3
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3
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4
1
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6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
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1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
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-
-
1
-
-
-
-
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2
-
4
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2
2
2
-
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1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
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1
-
-
-
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2
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4
1
-
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4
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1
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2
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3
1
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1
1
2
1
-
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1
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2
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4
1
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-
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1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
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1
1
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-
-
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3
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1
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2
1
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1
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1
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1
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2
1
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714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
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-
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2
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1
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2
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2
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1
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1
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1
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2
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1
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2
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1
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1
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1
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715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
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7
4
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2
3
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3
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1
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20
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18
1
1
1
1
4
1
1
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1
1
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7
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4
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2
3
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1
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20
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18
1
1
1
1
4
1
1
-
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-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
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1
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5
4
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2
3
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3
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1
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10
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13
1
1
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1
4
1
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1
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5
-
4
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2
3
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1
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10
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13
1
1
-
1
4
1
-
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4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
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1
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3
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3
1
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5
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1
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2
1
6
1
1
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1
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1
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3
1
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1
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2
1
6
1
1
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1
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1
-
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-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
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2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
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1
2
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5
1
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1
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1
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2
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1
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5
1
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1
1
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2
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1
1
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2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
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2
2
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5
1
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5
1
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1
1
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