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Literature summary for 5.3.1.15 extracted from

  • Choi, J.G.; Hong, S.H.; Kim, Y.S.; Kim, K.R.; Oh, D.K.
    Characterization of a recombinant thermostable D-lyxose isomerase from Dictyoglomus turgidum that produces D-lyxose from D-xylulose (2012), Biotechnol. Lett., 34, 1079-1085.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Dictyoglomus turgidum

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Dictyoglomus turgidum
EDTA
-
Dictyoglomus turgidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1 3 D-mannose at pH 7.5 and 75°C Dictyoglomus turgidum
5
-
D-xylulose at pH 7.5 and 75°C Dictyoglomus turgidum
39
-
D-Lyxose at pH 7.5 and 75°C Dictyoglomus turgidum
65
-
L-ribulose at pH 7.5 and 75°C Dictyoglomus turgidum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ maximum activity (6.6fold) in the presence of 0.5 mM Co2+ Dictyoglomus turgidum
Mn2+ stimulates activity but to a lesser extent than Co2+ Dictyoglomus turgidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
2 * 22000, SDS-PAGE Dictyoglomus turgidum
44000
-
gel filtration Dictyoglomus turgidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Lyxose Dictyoglomus turgidum highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h D-Xylulose
-
?
D-Lyxose Dictyoglomus turgidum DSM 6724 highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h D-Xylulose
-
?

Organism

Organism UniProt Comment Textmining
Dictyoglomus turgidum B8DZQ9
-
-
Dictyoglomus turgidum DSM 6724 B8DZQ9
-
-

Purification (Commentary)

Purification (Comment) Organism
metal ion affinity column chromatography Dictyoglomus turgidum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008
-
after 38fold purification, using L-allose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.022
-
after 38fold purification, using D-tagatose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.077
-
after 38fold purification, using D-ribulose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.094
-
after 38fold purification, using D-fructose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.106
-
after 38fold purification, using D-talose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.119
-
after 38fold purification, using L-ribose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.292
-
after 38fold purification, using L-ribulose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
0.5
-
crude extract, using D-lyxose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
1.668
-
after 38fold purification, using D-mannose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
18.8
-
after 38fold purification, using D-lyxose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum
33.1
-
after 38fold purification, using D-xylulose as substrate, at pH 7.5 and 75°C Dictyoglomus turgidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Dictyoglomus turgidum D-mannose
-
r
D-Lyxose
-
Dictyoglomus turgidum D-Xylulose
-
?
D-Lyxose highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h Dictyoglomus turgidum D-Xylulose
-
?
D-Lyxose
-
Dictyoglomus turgidum DSM 6724 D-Xylulose
-
?
D-Lyxose highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h Dictyoglomus turgidum DSM 6724 D-Xylulose
-
?
D-Mannose
-
Dictyoglomus turgidum D-Fructose
-
r
D-Mannose
-
Dictyoglomus turgidum DSM 6724 D-Fructose
-
r
D-ribulose
-
Dictyoglomus turgidum D-ribose
-
r
D-tagatose
-
Dictyoglomus turgidum D-talose
-
r
D-talose
-
Dictyoglomus turgidum D-tagatose
-
r
D-talose
-
Dictyoglomus turgidum DSM 6724 D-tagatose
-
r
D-xylulose
-
Dictyoglomus turgidum D-lyxose
-
r
L-allose
-
Dictyoglomus turgidum L-psicose
-
r
L-ribose
-
Dictyoglomus turgidum L-ribulose
-
r
L-ribose
-
Dictyoglomus turgidum DSM 6724 L-ribulose
-
r
L-ribulose
-
Dictyoglomus turgidum L-ribose
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 22000, SDS-PAGE Dictyoglomus turgidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Dictyoglomus turgidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 85 at pH 7.5 and 75°C and in the presence of 0.5 mM Co2+ the enzyme has a half-life of 108 min. After incubation at 75°C for 5 min, more than 97% of the enzyme activity remains. The half-lives of the enzyme at 60, 65, 70, 75, 80 and 85°C are 547, 247, 171, 108, 49, and 16 min, respectively Dictyoglomus turgidum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
L-ribulose at pH 7.5 and 75°C Dictyoglomus turgidum
2.97
-
D-mannose at pH 7.5 and 75°C Dictyoglomus turgidum
59.5
-
D-Lyxose at pH 7.5 and 75°C Dictyoglomus turgidum
69.3
-
D-xylulose at pH 7.5 and 75°C Dictyoglomus turgidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Dictyoglomus turgidum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
L-ribulose at pH 7.5 and 75°C Dictyoglomus turgidum
0.23
-
D-mannose at pH 7.5 and 75°C Dictyoglomus turgidum
1.52
-
D-Lyxose at pH 7.5 and 75°C Dictyoglomus turgidum
15.3
-
D-xylulose at pH 7.5 and 75°C Dictyoglomus turgidum