Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Dictyoglomus turgidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Dictyoglomus turgidum | |
EDTA | - |
Dictyoglomus turgidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 3 | D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
5 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
39 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
65 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | maximum activity (6.6fold) in the presence of 0.5 mM Co2+ | Dictyoglomus turgidum | |
Mn2+ | stimulates activity but to a lesser extent than Co2+ | Dictyoglomus turgidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Dictyoglomus turgidum |
44000 | - |
gel filtration | Dictyoglomus turgidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Lyxose | Dictyoglomus turgidum | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | D-Xylulose | - |
? | |
D-Lyxose | Dictyoglomus turgidum DSM 6724 | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | D-Xylulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus turgidum | B8DZQ9 | - |
- |
Dictyoglomus turgidum DSM 6724 | B8DZQ9 | - |
- |
Purification (Comment) | Organism |
---|---|
metal ion affinity column chromatography | Dictyoglomus turgidum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
after 38fold purification, using L-allose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.022 | - |
after 38fold purification, using D-tagatose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.077 | - |
after 38fold purification, using D-ribulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.094 | - |
after 38fold purification, using D-fructose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.106 | - |
after 38fold purification, using D-talose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.119 | - |
after 38fold purification, using L-ribose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.292 | - |
after 38fold purification, using L-ribulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
0.5 | - |
crude extract, using D-lyxose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
1.668 | - |
after 38fold purification, using D-mannose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
18.8 | - |
after 38fold purification, using D-lyxose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
33.1 | - |
after 38fold purification, using D-xylulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Dictyoglomus turgidum | D-mannose | - |
r | |
D-Lyxose | - |
Dictyoglomus turgidum | D-Xylulose | - |
? | |
D-Lyxose | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | Dictyoglomus turgidum | D-Xylulose | - |
? | |
D-Lyxose | - |
Dictyoglomus turgidum DSM 6724 | D-Xylulose | - |
? | |
D-Lyxose | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | Dictyoglomus turgidum DSM 6724 | D-Xylulose | - |
? | |
D-Mannose | - |
Dictyoglomus turgidum | D-Fructose | - |
r | |
D-Mannose | - |
Dictyoglomus turgidum DSM 6724 | D-Fructose | - |
r | |
D-ribulose | - |
Dictyoglomus turgidum | D-ribose | - |
r | |
D-tagatose | - |
Dictyoglomus turgidum | D-talose | - |
r | |
D-talose | - |
Dictyoglomus turgidum | D-tagatose | - |
r | |
D-talose | - |
Dictyoglomus turgidum DSM 6724 | D-tagatose | - |
r | |
D-xylulose | - |
Dictyoglomus turgidum | D-lyxose | - |
r | |
L-allose | - |
Dictyoglomus turgidum | L-psicose | - |
r | |
L-ribose | - |
Dictyoglomus turgidum | L-ribulose | - |
r | |
L-ribose | - |
Dictyoglomus turgidum DSM 6724 | L-ribulose | - |
r | |
L-ribulose | - |
Dictyoglomus turgidum | L-ribose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22000, SDS-PAGE | Dictyoglomus turgidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Dictyoglomus turgidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | at pH 7.5 and 75°C and in the presence of 0.5 mM Co2+ the enzyme has a half-life of 108 min. After incubation at 75°C for 5 min, more than 97% of the enzyme activity remains. The half-lives of the enzyme at 60, 65, 70, 75, 80 and 85°C are 547, 247, 171, 108, 49, and 16 min, respectively | Dictyoglomus turgidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
2.97 | - |
D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
59.5 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
69.3 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Dictyoglomus turgidum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
0.23 | - |
D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
1.52 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
15.3 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum |