BRENDA - Enzyme Database
show all sequences of 5.3.1.15

The structure of a D-lyxose isomerase from the deltaB regulon of Bacillus subtilis

Marles-Wright, J.; Lewis, R.; Proteins Struct. Funct. Bioinform. 79, 2015-2019 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli B834 (DE3) cells
Bacillus subtilis
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 0.2 M NaCl, 2.0 M ammonium sulfate
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P96578
-
-
Bacillus subtilis 168
P96578
-
-
Purification (Commentary)
Commentary
Organism
HiTrap column chromatography, Superdex S200 gel filtration
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
716872
Bacillus subtilis
D-Xylulose
-
-
-
?
D-Lyxose
-
716872
Bacillus subtilis 168
D-Xylulose
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
x-ray crystallography
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli B834 (DE3) cells
Bacillus subtilis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M sodium cacodylate pH 6.5, 0.2 M NaCl, 2.0 M ammonium sulfate
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
HiTrap column chromatography, Superdex S200 gel filtration
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
716872
Bacillus subtilis
D-Xylulose
-
-
-
?
D-Lyxose
-
716872
Bacillus subtilis 168
D-Xylulose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
x-ray crystallography
Bacillus subtilis
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
-
1
-
-
-
-
-
-
-
-
-
-
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6
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
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1
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-
-
-
-
-
-
-
-
-
747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
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-
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1
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1
1
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1
-
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-
-
-
-
-
-
749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
-
-
-
-
3
3
-
4
1
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-
2
-
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6
1
1
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3
3
1
1
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1
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3
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3
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4
1
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6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
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-
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3
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1
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2
1
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1
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1
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1
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2
1
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-
714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
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2
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2
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1
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1
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1
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2
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1
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2
-
1
-
1
-
1
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-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
4
1
1
-
-
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-
1
1
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-
-
7
-
4
-
2
3
-
-
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-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
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-
10
-
13
1
1
-
1
4
1
-
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-
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1
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-
-
-
5
-
4
-
2
3
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1
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-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
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-
5
-
-
1
-
-
-
2
1
6
1
1
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-
1
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1
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1
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3
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3
1
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1
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2
1
6
1
1
-
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-
1
-
-
1
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-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
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-
-
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2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
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1
2
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5
1
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1
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1
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1
1
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2
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1
1
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1
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2
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5
1
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1
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1
1
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2
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1
1
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-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
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2
2
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5
1
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1
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1
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2
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1
1
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2
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2
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5
1
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1
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2
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1
1
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