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Literature summary for 5.3.1.15 extracted from

  • Park, C.S.; Yeom, S.J.; Lim, Y.R.; Kim, Y.S.; Oh, D.K.
    Substrate specificity of a recombinant D-lyxose isomerase from Serratia proteamaculans that produces D-lyxose and D-mannose (2010), Lett. Appl. Microbiol., 51, 343-350.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli ER2566 cells Serratia proteamaculans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Serratia proteamaculans
EDTA
-
Serratia proteamaculans
Fe2+
-
Serratia proteamaculans
Ni2+
-
Serratia proteamaculans
Zn2+
-
Serratia proteamaculans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.83
-
D-xylulose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
13.3
-
D-Lyxose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
19.4
-
D-fructose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
32.2
-
D-mannose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the addition of Ca2+ ion to EDTA-treated enzyme enhances the activity of D-lyxose isomerization Serratia proteamaculans
Mn2+ maximum activity (190%) in the presence of 1 mM Mn2+ Serratia proteamaculans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26756
-
2 * 26756, calculated from amino acid sequence Serratia proteamaculans
27000
-
2 * 27000, SDS-PAGE Serratia proteamaculans
54000
-
gel filtration Serratia proteamaculans

Organism

Organism UniProt Comment Textmining
Serratia proteamaculans D5MTT1
-
-
Serratia proteamaculans KCTC 2936 D5MTT1
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Serratia proteamaculans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
using L-sorbose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
0.14
-
using L-ribose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
0.19
-
using D-talose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
0.28
-
using D-tagatose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
0.4
-
using L-ribulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
1.05
-
using L-gulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
1.41
-
using D-fructose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
5.42
-
using D-mannose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
10
-
using D-lyxose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
26.2
-
using D-xylulose as substrate, at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Serratia proteamaculans D-mannose
-
r
D-Lyxose highest activity Serratia proteamaculans D-Xylulose
-
r
D-Lyxose highest activity Serratia proteamaculans KCTC 2936 D-Xylulose
-
r
D-Mannose
-
Serratia proteamaculans D-Fructose
-
r
D-Mannose
-
Serratia proteamaculans KCTC 2936 D-Fructose
-
r
D-talose
-
Serratia proteamaculans D-tagatose
-
r
D-talose
-
Serratia proteamaculans KCTC 2936 D-tagatose
-
r
D-xylulose
-
Serratia proteamaculans D-lyxose
-
r
L-gulose
-
Serratia proteamaculans L-sorbose
-
r
L-ribose
-
Serratia proteamaculans L-ribulose
-
r
L-ribose
-
Serratia proteamaculans KCTC 2936 L-ribulose
-
r
additional information no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Serratia proteamaculans ?
-
?
additional information no activity with D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate Serratia proteamaculans KCTC 2936 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 27000, SDS-PAGE Serratia proteamaculans
homodimer 2 * 26756, calculated from amino acid sequence Serratia proteamaculans

Synonyms

Synonyms Comment Organism
D-Lyxose isomerase
-
Serratia proteamaculans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Serratia proteamaculans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme shows half-lives of 84 h, 17 h, 2.6 h, 0.3 h, and 0.09 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively Serratia proteamaculans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4297
-
D-fructose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
16170
-
D-mannose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
30810
-
D-Lyxose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
31620
-
D-xylulose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Serratia proteamaculans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
222
-
D-fructose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
502
-
D-mannose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
2333
-
D-Lyxose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans
9560
-
D-xylulose at pH 7.5 and 40°C, in the presence of 1 mM Mn2+ Serratia proteamaculans