Application | Comment | Organism |
---|---|---|
food industry | the enzyme is an industrial producer of D-lyxose | Providencia stuartii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Providencia stuartii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 80% residual activity at 1 mM | Providencia stuartii | |
Co2+ | about 15% residual activity at 1 mM | Providencia stuartii | |
Cu2+ | about 55% residual activity at 1 mM | Providencia stuartii | |
EDTA | 12% residual activity at 1 mM | Providencia stuartii | |
Fe2+ | about 60% residual activity at 1 mM | Providencia stuartii | |
additional information | not affected by Ca2+ | Providencia stuartii | |
Zn2+ | about 60% residual activity at 1 mM | Providencia stuartii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
D-mannose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
38 | - |
D-xylulose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
47 | - |
D-Lyxose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
54 | - |
D-fructose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | about 150% activity at 1 mM Mg2+ | Providencia stuartii | |
Mn2+ | maximal activity (about 250%) in the presence of 1 mM Mn2+ | Providencia stuartii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
2 * 22000, SDS-PAGE | Providencia stuartii |
22160 | - |
2 * 22160, calculated from amino acid sequence | Providencia stuartii |
44000 | - |
gel filtration | Providencia stuartii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia stuartii | - |
- |
- |
Providencia stuartii KCTC 2568 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography, gel filtration | Providencia stuartii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.002 | - |
using L-tagatose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.003 | - |
using L-mannose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.005 | - |
using D-psicose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.007 | - |
using L-lyxose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.01 | - |
using L-psicose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.011 | - |
using L-talose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.013 | - |
using D-allose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.014 | - |
using D-ribose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.015 | - |
using D-tagatose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.017 | - |
using L-fructose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.022 | - |
using L-allose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.045 | - |
using D-tagatose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.096 | - |
using D-ribulose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.18 | - |
using L-xylulose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.219 | - |
using L-ribose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.403 | - |
using D-fructose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.523 | - |
using D-mannose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
0.872 | - |
using L-ribulose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
22.33 | - |
using D-lyxose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
24.04 | - |
using D-xylulose as substrate, at pH 7.5 and 45°C | Providencia stuartii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Providencia stuartii | D-psicose | - |
r | |
D-allose | - |
Providencia stuartii KCTC 2568 | D-psicose | - |
r | |
D-fructose | - |
Providencia stuartii | D-mannose | - |
r | |
D-Lyxose | highest specific activity | Providencia stuartii | D-Xylulose | - |
r | |
D-Mannose | - |
Providencia stuartii | D-Fructose | - |
r | |
D-ribose | - |
Providencia stuartii | D-ribulose | - |
r | |
D-ribose | - |
Providencia stuartii KCTC 2568 | D-ribulose | - |
r | |
D-talose | - |
Providencia stuartii | D-tagatose | - |
r | |
D-xylulose | - |
Providencia stuartii | D-lyxose | - |
r | |
L-allose | - |
Providencia stuartii | L-psicose | - |
r | |
L-lyxose | - |
Providencia stuartii | L-xylulose | - |
r | |
L-lyxose | - |
Providencia stuartii KCTC 2568 | L-xylulose | - |
r | |
L-Mannose | - |
Providencia stuartii | L-Fructose | - |
r | |
L-Mannose | - |
Providencia stuartii KCTC 2568 | L-Fructose | - |
r | |
L-ribose | - |
Providencia stuartii | L-ribulose | - |
r | |
L-talose | - |
Providencia stuartii | L-tagatose | - |
r | |
additional information | the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Providencia stuartii | ? | - |
? | |
additional information | the enzyme does not isomerizes D-mannose 6-phosphate, D-glucose 6-phosphate, and D-ribose 6-phosphate | Providencia stuartii KCTC 2568 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 22000, SDS-PAGE | Providencia stuartii |
homodimer | 2 * 22160, calculated from amino acid sequence | Providencia stuartii |
Synonyms | Comment | Organism |
---|---|---|
D-Lyxose isomerase | - |
Providencia stuartii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Providencia stuartii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | at 40°C and 50°C, the activities are approximately 60% of the maximum | Providencia stuartii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme shows half-lives of 36 h, 14 h, 8.9 h, 3.4 h, and 1.4 h at 30°C, 35°C, 40°C, 45°C, and 50 °C, respectively | Providencia stuartii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2010 | - |
D-fructose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
2640 | - |
D-mannose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
31340 | - |
D-Lyxose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
34960 | - |
D-xylulose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Providencia stuartii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | at pH 7.0 and 8.5, the activities are approximately 60% of the maximum | Providencia stuartii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
D-fructose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
116 | - |
D-mannose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
669 | - |
D-Lyxose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii | |
920 | - |
D-xylulose | in 50 mM HEPES buffer, at pH 7.5, 45°C | Providencia stuartii |