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Literature summary for 5.3.1.15 extracted from

  • Park, C.S.; Kwon, H.J.; Yeom, S.J.; Oh, D.K.
    Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartii (2010), Biotechnol. Lett., 32, 1305-1309.
    View publication on PubMed

General Stability

General Stability Organism
approximately 10% of the total activity is lost during theimmobilization process on Duolite A568 beads. The Providencia stuartii
conversion yields using Duolite A568 and Chitopearl BCW2510 beads are 25 and 16%, respectively. Those using Amberlite IRA-120 S and IRA-400 ZLB beads are 7 and 9%, respectively. Immobilization on XAD7, XAD8, and Sephadex LH-60-120 results in no conversion Providencia stuartii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ optimum activity in the presence of 1 mM Mn2+ Providencia stuartii

Organism

Organism UniProt Comment Textmining
Providencia stuartii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose
-
Providencia stuartii D-mannose
-
?
D-Lyxose
-
Providencia stuartii D-Xylulose
-
?

Synonyms

Synonyms Comment Organism
D-Lyxose isomerase
-
Providencia stuartii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
enzyme immobilized on Duolite A568 beads Providencia stuartii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the half-lives of the free enzyme at 35, 40, 45, and 50°C are 14 h, 8.9 h, 3.4 h, and 1.4 h, while those of the enzyme immobilized on Duolite A568 are 30 h, 15 h, 5.1 h, and 2.2 h, respectively Providencia stuartii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
enzyme immobilized on Duolite A568 beads Providencia stuartii