BRENDA - Enzyme Database show
show all sequences of 5.3.1.15

Mannose production from fructose by free and immobilized D-lyxose isomerases from Providencia stuartii

Park, C.S.; Kwon, H.J.; Yeom, S.J.; Oh, D.K.; Biotechnol. Lett. 32, 1305-1309 (2010)

Data extracted from this reference:

General Stability
General Stability
Organism
approximately 10% of the total activity is lost during theimmobilization process on Duolite A568 beads. The
Providencia stuartii
conversion yields using Duolite A568 and Chitopearl BCW2510 beads are 25 and 16%, respectively. Those using Amberlite IRA-120 S and IRA-400 ZLB beads are 7 and 9%, respectively. Immobilization on XAD7, XAD8, and Sephadex LH-60-120 results in no conversion
Providencia stuartii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
optimum activity in the presence of 1 mM Mn2+
Providencia stuartii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Providencia stuartii
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
-
714568
Providencia stuartii
D-mannose
-
-
-
?
D-Lyxose
-
714568
Providencia stuartii
D-Xylulose
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
enzyme immobilized on Duolite A568 beads
Providencia stuartii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
the half-lives of the free enzyme at 35, 40, 45, and 50°C are 14 h, 8.9 h, 3.4 h, and 1.4 h, while those of the enzyme immobilized on Duolite A568 are 30 h, 15 h, 5.1 h, and 2.2 h, respectively
Providencia stuartii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
enzyme immobilized on Duolite A568 beads
Providencia stuartii
General Stability (protein specific)
General Stability
Organism
approximately 10% of the total activity is lost during theimmobilization process on Duolite A568 beads. The
Providencia stuartii
conversion yields using Duolite A568 and Chitopearl BCW2510 beads are 25 and 16%, respectively. Those using Amberlite IRA-120 S and IRA-400 ZLB beads are 7 and 9%, respectively. Immobilization on XAD7, XAD8, and Sephadex LH-60-120 results in no conversion
Providencia stuartii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
optimum activity in the presence of 1 mM Mn2+
Providencia stuartii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
-
714568
Providencia stuartii
D-mannose
-
-
-
?
D-Lyxose
-
714568
Providencia stuartii
D-Xylulose
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
enzyme immobilized on Duolite A568 beads
Providencia stuartii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
the half-lives of the free enzyme at 35, 40, 45, and 50°C are 14 h, 8.9 h, 3.4 h, and 1.4 h, while those of the enzyme immobilized on Duolite A568 are 30 h, 15 h, 5.1 h, and 2.2 h, respectively
Providencia stuartii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
enzyme immobilized on Duolite A568 beads
Providencia stuartii
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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Cohnella laeviribosi
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97
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1
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-
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4
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1
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1
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1
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749201
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-
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2026-2033
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1
-
-
-
-
3
3
-
4
1
-
-
2
-
-
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-
-
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6
1
1
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3
3
1
1
-
-
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-
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1
-
-
-
-
-
-
3
-
3
-
4
1
-
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
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1
-
-
-
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-
2
1
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-
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1
-
1
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1
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-
2
1
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-
-
-
-
-
-
-
-
-
-
-
-
714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
7
-
4
-
2
3
-
-
-
-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
-
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
2
3
-
-
-
-
1
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
-
-
5
-
-
1
-
-
-
2
1
6
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
-
-
1
-
-
2
1
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
-
-
-
-
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-
-
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2
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5
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5
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
-
-
1
2
-
5
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
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1
-
2
-
5
1
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
-
-
-
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2
2
-
5
1
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1
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1
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-
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2
-
-
-
-
-
1
1
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2
-
2
-
5
1
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1
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2
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1
1
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