BRENDA - Enzyme Database show
show all sequences of 5.3.1.15

Substrate specificity of the Bacillus licheniformis lyxose isomerase YdaE and its application in in vitro catalysis for bioproduction of lyxose and glucose by two-step isomerization

Patel, D.; Wi, S.; Lee, S.; Lee, D.; Song, Y.; Bae, H.; Appl. Environ. Microbiol. 77, 3343-3350 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus licheniformis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
30.4
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
about 40% activity in the presence of 1 mM Co2+ as compared to Mn2+
Bacillus licheniformis
Mg2+
about 50% activity in the presence of 1 mM Mg2+ as compared to Mn2+
Bacillus licheniformis
Mn2+
highest activity (100%) in the presence of 1.0 mM Mn2+
Bacillus licheniformis
additional information
not activated by Zn2+, Cu2+, and Ni2+
Bacillus licheniformis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19500
-
x * 19500, estimated from SDS-PAGE
Bacillus licheniformis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus licheniformis
Q65N98
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose bead column chromatography and ultrafiltration
Bacillus licheniformis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
crude extract, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
24
-
after 10fold purification, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
713845
Bacillus licheniformis
D-Xylulose
-
-
-
r
D-Mannose
-
713845
Bacillus licheniformis
?
-
-
-
?
additional information
no activity with D-glucose, D-xylose, L-arabinose, and L-rhamnose
713845
Bacillus licheniformis
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 19500, estimated from SDS-PAGE
Bacillus licheniformis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
65
the enzyme activity increases as the temperature increases, showing higher activity at 40°C to 45°C, and is completely lost at temperatures greater than 65°C
Bacillus licheniformis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
the enzyme shows half-lives of 140 h, 62 h, 30 h, 18 h, and 7 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively
Bacillus licheniformis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
43
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
98
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Bacillus licheniformis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Bacillus licheniformis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
30.4
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
about 40% activity in the presence of 1 mM Co2+ as compared to Mn2+
Bacillus licheniformis
Mg2+
about 50% activity in the presence of 1 mM Mg2+ as compared to Mn2+
Bacillus licheniformis
Mn2+
highest activity (100%) in the presence of 1.0 mM Mn2+
Bacillus licheniformis
additional information
not activated by Zn2+, Cu2+, and Ni2+
Bacillus licheniformis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19500
-
x * 19500, estimated from SDS-PAGE
Bacillus licheniformis
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose bead column chromatography and ultrafiltration
Bacillus licheniformis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
crude extract, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
24
-
after 10fold purification, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
713845
Bacillus licheniformis
D-Xylulose
-
-
-
r
D-Mannose
-
713845
Bacillus licheniformis
?
-
-
-
?
additional information
no activity with D-glucose, D-xylose, L-arabinose, and L-rhamnose
713845
Bacillus licheniformis
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 19500, estimated from SDS-PAGE
Bacillus licheniformis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
40
65
the enzyme activity increases as the temperature increases, showing higher activity at 40°C to 45°C, and is completely lost at temperatures greater than 65°C
Bacillus licheniformis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
50
the enzyme shows half-lives of 140 h, 62 h, 30 h, 18 h, and 7 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively
Bacillus licheniformis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
43
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
98
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
-
Bacillus licheniformis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.6
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
3.2
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.6
-
D-mannose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
3.2
-
D-Lyxose
in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C
Bacillus licheniformis
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
-
-
-
-
3
3
-
4
1
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
4
1
-
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
7
-
4
-
2
3
-
-
-
-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
-
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
2
3
-
-
-
-
1
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
-
-
5
-
-
1
-
-
-
2
1
6
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
-
-
1
-
-
2
1
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
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-
5
-
-
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5
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-
-
-
-
-
-
-
-
2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
-
-
1
2
-
5
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
-
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1
-
2
-
5
1
-
-
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1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
-
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2
2
-
5
1
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1
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1
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2
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1
1
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2
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2
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5
1
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1
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2
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1
1
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