Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
D-mannose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis | |
30.4 | - |
D-Lyxose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | about 40% activity in the presence of 1 mM Co2+ as compared to Mn2+ | Bacillus licheniformis | |
Mg2+ | about 50% activity in the presence of 1 mM Mg2+ as compared to Mn2+ | Bacillus licheniformis | |
Mn2+ | highest activity (100%) in the presence of 1.0 mM Mn2+ | Bacillus licheniformis | |
additional information | not activated by Zn2+, Cu2+, and Ni2+ | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19500 | - |
x * 19500, estimated from SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65N98 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose bead column chromatography and ultrafiltration | Bacillus licheniformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.2 | - |
crude extract, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis |
24 | - |
after 10fold purification, in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Lyxose | - |
Bacillus licheniformis | D-Xylulose | - |
r | |
D-Mannose | - |
Bacillus licheniformis | ? | - |
? | |
additional information | no activity with D-glucose, D-xylose, L-arabinose, and L-rhamnose | Bacillus licheniformis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19500, estimated from SDS-PAGE | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
D-Lyxose isomerase | - |
Bacillus licheniformis |
YdaE | - |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | the enzyme activity increases as the temperature increases, showing higher activity at 40°C to 45°C, and is completely lost at temperatures greater than 65°C | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | the enzyme shows half-lives of 140 h, 62 h, 30 h, 18 h, and 7 h at 30°C, 35°C, 40°C, 45°C, and 50°C, respectively | Bacillus licheniformis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
D-mannose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis | |
98 | - |
D-Lyxose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
D-mannose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis | |
3.2 | - |
D-Lyxose | in 50 mM Tris-HCl, 1 mM Mn2+, pH 8.0, at 40°C | Bacillus licheniformis |