BRENDA - Enzyme Database
show all sequences of 5.3.1.15

Characterization of a novel D-lyxose isomerase from Cohnella laevoribosii RI-39 sp. nov

Cho, E.A.; Lee, D.W.; Cha, Y.H.; Lee, S.J.; Jung, H.C.; Pan, J.G.; Pyun, Y.R.; J. Bacteriol. 189, 1655-1663 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Escherichia coli
Cohnella laeviribosi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
22.4
-
D-Lyxose
-
Cohnella laeviribosi
34
-
D-mannose
-
Cohnella laeviribosi
121.7
-
L-ribose
-
Cohnella laeviribosi
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
activating at 1 mM, D-xylose as substrate
Cohnella laeviribosi
Mn2+
at 1 mM, D-xylose as substrate, requires divalent metal ions
Cohnella laeviribosi
Ni2+
activating at 1 mM, D-xylose as substrate
Cohnella laeviribosi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Cohnella laeviribosi
Organism
Organism
UniProt
Commentary
Textmining
Cohnella laeviribosi
A3E7Z6
-
-
Cohnella laeviribosi RI-39
A3E7Z6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
of the native protein to homogeneity by DEAE-Sephacel ion-exchange chromatography, followed by RESOURCE PHE hydrophobic chromatography, of the recombinant protein
Cohnella laeviribosi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.4
-
of the native enzyme after purification, at 70C, with L-ribose as substrate
Cohnella laeviribosi
50.2
-
of the recombinant enzyme after purification, at 70C, with L-ribose as substrate
Cohnella laeviribosi
Storage Stability
Storage Stability
Organism
at -20C for several months
Cohnella laeviribosi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-Lyxose
-
674319
Cohnella laeviribosi
D-Xylulose
-
-
-
r
D-Lyxose
-
674319
Cohnella laeviribosi RI-39
D-Xylulose
-
-
-
r
D-Mannose
no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate
674319
Cohnella laeviribosi
D-Fructose
-
-
-
r
D-Mannose
no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate
674319
Cohnella laeviribosi RI-39
D-Fructose
-
-
-
r
L-ribose
-
674319
Cohnella laeviribosi
L-ribulose
-
-
-
r
L-ribose
-
674319
Cohnella laeviribosi RI-39
L-ribulose
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 x 21000 SDS-PAGE
Cohnella laeviribosi
Synonyms
Synonyms
Commentary
Organism
CLLI
D-lyxose isomerase from Cohnella laevoribosii
Cohnella laeviribosi
CLRI
L-ribose isomerase from Cohnella laevoribosii
Cohnella laeviribosi
D-Lyxose isomerase
-
Cohnella laeviribosi
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
wild-type and recombinant enzymes at 1 mM Mn2+
Cohnella laeviribosi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
wild-type and recombinant enzymes at 1 mM Mn2+
Cohnella laeviribosi
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Cohnella laeviribosi
wild-type and recombinant enzymes
-
4.2
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Cohnella laeviribosi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
22.4
-
D-Lyxose
-
Cohnella laeviribosi
34
-
D-mannose
-
Cohnella laeviribosi
121.7
-
L-ribose
-
Cohnella laeviribosi
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
activating at 1 mM, D-xylose as substrate
Cohnella laeviribosi
Mn2+
at 1 mM, D-xylose as substrate, requires divalent metal ions
Cohnella laeviribosi
Ni2+
activating at 1 mM, D-xylose as substrate
Cohnella laeviribosi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Cohnella laeviribosi
Purification (Commentary) (protein specific)
Commentary
Organism
of the native protein to homogeneity by DEAE-Sephacel ion-exchange chromatography, followed by RESOURCE PHE hydrophobic chromatography, of the recombinant protein
Cohnella laeviribosi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.4
-
of the native enzyme after purification, at 70C, with L-ribose as substrate
Cohnella laeviribosi
50.2
-
of the recombinant enzyme after purification, at 70C, with L-ribose as substrate
Cohnella laeviribosi
Storage Stability (protein specific)
Storage Stability
Organism
at -20C for several months
Cohnella laeviribosi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-Lyxose
-
674319
Cohnella laeviribosi
D-Xylulose
-
-
-
r
D-Lyxose
-
674319
Cohnella laeviribosi RI-39
D-Xylulose
-
-
-
r
D-Mannose
no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate
674319
Cohnella laeviribosi
D-Fructose
-
-
-
r
D-Mannose
no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate
674319
Cohnella laeviribosi RI-39
D-Fructose
-
-
-
r
L-ribose
-
674319
Cohnella laeviribosi
L-ribulose
-
-
-
r
L-ribose
-
674319
Cohnella laeviribosi RI-39
L-ribulose
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 x 21000 SDS-PAGE
Cohnella laeviribosi
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
wild-type and recombinant enzymes at 1 mM Mn2+
Cohnella laeviribosi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
wild-type and recombinant enzymes at 1 mM Mn2+
Cohnella laeviribosi
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Cohnella laeviribosi
wild-type and recombinant enzymes
-
4.2
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
-
-
-
-
3
3
-
4
1
-
-
2
-
-
-
-
-
-
-
-
6
1
-
1
-
3
3
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
4
1
-
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
-
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
2
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
1
4
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
7
-
4
-
2
3
-
-
-
-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
-
-
-
10
-
13
1
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
2
3
-
-
-
-
1
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
-
-
5
-
-
1
-
-
-
2
1
6
1
3
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
-
-
1
-
-
2
1
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
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-
-
5
-
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5
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-
-
-
-
-
-
-
-
-
-
-
-
-
2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
-
-
1
2
-
5
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
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1
1
-
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-
-
-
-
-
-
-
-
-
-
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1
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2
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5
1
-
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-
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1
-
1
1
-
2
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
-
-
-
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2
2
-
5
1
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1
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1
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2
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1
1
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2
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2
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5
1
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1
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2
-
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1
1
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