Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Cohnella laeviribosi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22.4 | - |
D-Lyxose | - |
Cohnella laeviribosi | |
34 | - |
D-mannose | - |
Cohnella laeviribosi | |
121.7 | - |
L-ribose | - |
Cohnella laeviribosi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activating at 1 mM, D-xylose as substrate | Cohnella laeviribosi | |
Mn2+ | at 1 mM, D-xylose as substrate, requires divalent metal ions | Cohnella laeviribosi | |
Ni2+ | activating at 1 mM, D-xylose as substrate | Cohnella laeviribosi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Cohnella laeviribosi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cohnella laeviribosi | A3E7Z6 | - |
- |
Cohnella laeviribosi RI-39 | A3E7Z6 | - |
- |
Purification (Comment) | Organism |
---|---|
of the native protein to homogeneity by DEAE-Sephacel ion-exchange chromatography, followed by RESOURCE PHE hydrophobic chromatography, of the recombinant protein | Cohnella laeviribosi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.4 | - |
of the native enzyme after purification, at 70°C, with L-ribose as substrate | Cohnella laeviribosi |
50.2 | - |
of the recombinant enzyme after purification, at 70°C, with L-ribose as substrate | Cohnella laeviribosi |
Storage Stability | Organism |
---|---|
at -20°C for several months | Cohnella laeviribosi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Lyxose | - |
Cohnella laeviribosi | D-Xylulose | - |
r | |
D-Lyxose | - |
Cohnella laeviribosi RI-39 | D-Xylulose | - |
r | |
D-Mannose | no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate | Cohnella laeviribosi | D-Fructose | - |
r | |
D-Mannose | no activity with L-lyxose, Dxylose,L-xylose, D-arabinose, L-arabinose, D-ribose, L-fucose, D-glucose, D-galactose, D-erythrose, L-rhamnose, 2-deoxy-D-ribose, and ribose-5-phosphate | Cohnella laeviribosi RI-39 | D-Fructose | - |
r | |
L-ribose | - |
Cohnella laeviribosi | L-ribulose | - |
r | |
L-ribose | - |
Cohnella laeviribosi RI-39 | L-ribulose | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 x 21000 SDS-PAGE | Cohnella laeviribosi |
Synonyms | Comment | Organism |
---|---|---|
CLLI | D-lyxose isomerase from Cohnella laevoribosii | Cohnella laeviribosi |
CLRI | L-ribose isomerase from Cohnella laevoribosii | Cohnella laeviribosi |
D-Lyxose isomerase | - |
Cohnella laeviribosi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
wild-type and recombinant enzymes at 1 mM Mn2+ | Cohnella laeviribosi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
wild-type and recombinant enzymes at 1 mM Mn2+ | Cohnella laeviribosi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cohnella laeviribosi | wild-type and recombinant enzymes | - |
4.2 |