BRENDA - Enzyme Database show
show all sequences of 5.3.1.15

D-Lyxose isomerase

Anderson, R.L.; Methods Enzymol. 9, 593-596 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
PCMB
-
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
D-Lyxose
-
Klebsiella aerogenes
10
-
mannose
-
Klebsiella aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
metal ion required, Ca2+ activates slightly
Klebsiella aerogenes
Co2+
metal ion required, Co2+ activates slightly
Klebsiella aerogenes
Fe2+
metal ion required, Fe2+ activates
Klebsiella aerogenes
Fe3+
metal ion required, Fe3+ activates
Klebsiella aerogenes
Mn2+
metal ion required, Mn2+ activates most effectively. Km: 0.0004 mM
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration, density gradient centrifugation
Klebsiella aerogenes
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
-
-
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella aerogenes
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:D-lyxose-grown cell
-
Klebsiella aerogenes
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.7
-
-
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
2639
Klebsiella aerogenes
D-Xylulose
-
-
-
-
D-Mannose
-
2639
Klebsiella aerogenes
D-Fructose
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Klebsiella aerogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.9
7
pH 4.0: about 50% of maximal activity, pH 7.0: maximal activity
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PCMB
-
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
D-Lyxose
-
Klebsiella aerogenes
10
-
mannose
-
Klebsiella aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
metal ion required, Ca2+ activates slightly
Klebsiella aerogenes
Co2+
metal ion required, Co2+ activates slightly
Klebsiella aerogenes
Fe2+
metal ion required, Fe2+ activates
Klebsiella aerogenes
Fe3+
metal ion required, Fe3+ activates
Klebsiella aerogenes
Mn2+
metal ion required, Mn2+ activates most effectively. Km: 0.0004 mM
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
gel filtration, density gradient centrifugation
Klebsiella aerogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella aerogenes
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:D-lyxose-grown cell
-
Klebsiella aerogenes
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
7.7
-
-
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Lyxose
-
2639
Klebsiella aerogenes
D-Xylulose
-
-
-
-
D-Mannose
-
2639
Klebsiella aerogenes
D-Fructose
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Klebsiella aerogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.9
7
pH 4.0: about 50% of maximal activity, pH 7.0: maximal activity
Klebsiella aerogenes
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
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1
1
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
-
-
-
-
3
3
-
4
1
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
4
1
-
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
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1
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-
-
-
2
1
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-
-
-
-
-
-
-
-
-
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-
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714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
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1
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-
-
-
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-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
7
-
4
-
2
3
-
-
-
-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
-
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
2
3
-
-
-
-
1
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
-
-
5
-
-
1
-
-
-
2
1
6
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
-
-
1
-
-
2
1
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
-
-
-
-
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-
-
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2
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5
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5
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-
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-
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-
2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
-
-
1
2
-
5
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
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-
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-
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1
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2
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5
1
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1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
-
-
-
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2
2
-
5
1
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1
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1
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-
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2
-
-
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1
1
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2
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2
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5
1
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1
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2
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1
1
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