BRENDA - Enzyme Database
show all sequences of 5.3.1.15

Characterization of a recombinant thermostable D-lyxose isomerase from Dictyoglomus turgidum that produces D-lyxose from D-xylulose

Choi, J.G.; Hong, S.H.; Kim, Y.S.; Kim, K.R.; Oh, D.K.; Biotechnol. Lett. 34, 1079-1085 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Dictyoglomus turgidum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Dictyoglomus turgidum
EDTA
-
Dictyoglomus turgidum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
3
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
5
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
39
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
65
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
maximum activity (6.6fold) in the presence of 0.5 mM Co2+
Dictyoglomus turgidum
Mn2+
stimulates activity but to a lesser extent than Co2+
Dictyoglomus turgidum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
2 * 22000, SDS-PAGE
Dictyoglomus turgidum
44000
-
gel filtration
Dictyoglomus turgidum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-Lyxose
Dictyoglomus turgidum
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
D-Xylulose
-
-
?
D-Lyxose
Dictyoglomus turgidum DSM 6724
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
D-Xylulose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Dictyoglomus turgidum
B8DZQ9
-
-
Dictyoglomus turgidum DSM 6724
B8DZQ9
-
-
Purification (Commentary)
Commentary
Organism
metal ion affinity column chromatography
Dictyoglomus turgidum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.008
-
after 38fold purification, using L-allose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.022
-
after 38fold purification, using D-tagatose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.077
-
after 38fold purification, using D-ribulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.094
-
after 38fold purification, using D-fructose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.106
-
after 38fold purification, using D-talose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.119
-
after 38fold purification, using L-ribose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.292
-
after 38fold purification, using L-ribulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.5
-
crude extract, using D-lyxose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
1.668
-
after 38fold purification, using D-mannose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
18.8
-
after 38fold purification, using D-lyxose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
33.1
-
after 38fold purification, using D-xylulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
-
727225
Dictyoglomus turgidum
D-mannose
-
-
-
r
D-Lyxose
-
727225
Dictyoglomus turgidum
D-Xylulose
-
-
-
?
D-Lyxose
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
727225
Dictyoglomus turgidum
D-Xylulose
-
-
-
?
D-Lyxose
-
727225
Dictyoglomus turgidum DSM 6724
D-Xylulose
-
-
-
?
D-Lyxose
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
727225
Dictyoglomus turgidum DSM 6724
D-Xylulose
-
-
-
?
D-Mannose
-
727225
Dictyoglomus turgidum
D-Fructose
-
-
-
r
D-Mannose
-
727225
Dictyoglomus turgidum DSM 6724
D-Fructose
-
-
-
r
D-ribulose
-
727225
Dictyoglomus turgidum
D-ribose
-
-
-
r
D-tagatose
-
727225
Dictyoglomus turgidum
D-talose
-
-
-
r
D-talose
-
727225
Dictyoglomus turgidum
D-tagatose
-
-
-
r
D-talose
-
727225
Dictyoglomus turgidum DSM 6724
D-tagatose
-
-
-
r
D-xylulose
-
727225
Dictyoglomus turgidum
D-lyxose
-
-
-
r
L-allose
-
727225
Dictyoglomus turgidum
L-psicose
-
-
-
r
L-ribose
-
727225
Dictyoglomus turgidum
L-ribulose
-
-
-
r
L-ribose
-
727225
Dictyoglomus turgidum DSM 6724
L-ribulose
-
-
-
r
L-ribulose
-
727225
Dictyoglomus turgidum
L-ribose
-
-
-
r
Subunits
Subunits
Commentary
Organism
homodimer
2 * 22000, SDS-PAGE
Dictyoglomus turgidum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
75
-
-
Dictyoglomus turgidum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
85
at pH 7.5 and 75°C and in the presence of 0.5 mM Co2+ the enzyme has a half-life of 108 min. After incubation at 75°C for 5 min, more than 97% of the enzyme activity remains. The half-lives of the enzyme at 60, 65, 70, 75, 80 and 85°C are 547, 247, 171, 108, 49, and 16 min, respectively
Dictyoglomus turgidum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
2.97
-
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
59.5
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
69.3
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Dictyoglomus turgidum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Dictyoglomus turgidum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Dictyoglomus turgidum
EDTA
-
Dictyoglomus turgidum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
3
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
5
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
39
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
65
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
maximum activity (6.6fold) in the presence of 0.5 mM Co2+
Dictyoglomus turgidum
Mn2+
stimulates activity but to a lesser extent than Co2+
Dictyoglomus turgidum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
2 * 22000, SDS-PAGE
Dictyoglomus turgidum
44000
-
gel filtration
Dictyoglomus turgidum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-Lyxose
Dictyoglomus turgidum
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
D-Xylulose
-
-
?
D-Lyxose
Dictyoglomus turgidum DSM 6724
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
D-Xylulose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
metal ion affinity column chromatography
Dictyoglomus turgidum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.008
-
after 38fold purification, using L-allose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.022
-
after 38fold purification, using D-tagatose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.077
-
after 38fold purification, using D-ribulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.094
-
after 38fold purification, using D-fructose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.106
-
after 38fold purification, using D-talose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.119
-
after 38fold purification, using L-ribose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.292
-
after 38fold purification, using L-ribulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
0.5
-
crude extract, using D-lyxose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
1.668
-
after 38fold purification, using D-mannose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
18.8
-
after 38fold purification, using D-lyxose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
33.1
-
after 38fold purification, using D-xylulose as substrate, at pH 7.5 and 75°C
Dictyoglomus turgidum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-fructose
-
727225
Dictyoglomus turgidum
D-mannose
-
-
-
r
D-Lyxose
-
727225
Dictyoglomus turgidum
D-Xylulose
-
-
-
?
D-Lyxose
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
727225
Dictyoglomus turgidum
D-Xylulose
-
-
-
?
D-Lyxose
-
727225
Dictyoglomus turgidum DSM 6724
D-Xylulose
-
-
-
?
D-Lyxose
highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h
727225
Dictyoglomus turgidum DSM 6724
D-Xylulose
-
-
-
?
D-Mannose
-
727225
Dictyoglomus turgidum
D-Fructose
-
-
-
r
D-Mannose
-
727225
Dictyoglomus turgidum DSM 6724
D-Fructose
-
-
-
r
D-ribulose
-
727225
Dictyoglomus turgidum
D-ribose
-
-
-
r
D-tagatose
-
727225
Dictyoglomus turgidum
D-talose
-
-
-
r
D-talose
-
727225
Dictyoglomus turgidum
D-tagatose
-
-
-
r
D-talose
-
727225
Dictyoglomus turgidum DSM 6724
D-tagatose
-
-
-
r
D-xylulose
-
727225
Dictyoglomus turgidum
D-lyxose
-
-
-
r
L-allose
-
727225
Dictyoglomus turgidum
L-psicose
-
-
-
r
L-ribose
-
727225
Dictyoglomus turgidum
L-ribulose
-
-
-
r
L-ribose
-
727225
Dictyoglomus turgidum DSM 6724
L-ribulose
-
-
-
r
L-ribulose
-
727225
Dictyoglomus turgidum
L-ribose
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 22000, SDS-PAGE
Dictyoglomus turgidum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
75
-
-
Dictyoglomus turgidum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
85
at pH 7.5 and 75°C and in the presence of 0.5 mM Co2+ the enzyme has a half-life of 108 min. After incubation at 75°C for 5 min, more than 97% of the enzyme activity remains. The half-lives of the enzyme at 60, 65, 70, 75, 80 and 85°C are 547, 247, 171, 108, 49, and 16 min, respectively
Dictyoglomus turgidum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
2.97
-
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
59.5
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
69.3
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Dictyoglomus turgidum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
0.23
-
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
1.52
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
15.3
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.03
-
L-ribulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
0.23
-
D-mannose
at pH 7.5 and 75°C
Dictyoglomus turgidum
1.52
-
D-Lyxose
at pH 7.5 and 75°C
Dictyoglomus turgidum
15.3
-
D-xylulose
at pH 7.5 and 75°C
Dictyoglomus turgidum
Other publictions for EC 5.3.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748502
Huang
Production of D-mannose from ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 16646
J. Sci. Food. Agric.
98
4895-4902
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747682
Patel
A single and two step isomeri ...
Cohnella laeviribosi
Enzyme Microb. Technol.
97
27-33
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749201
Yu
-
Efficient biotransformation o ...
Thermosediminibacter oceani, Thermosediminibacter oceani DSM 1664
Process Biochem.
51
2026-2033
2016
-
1
-
-
-
-
3
3
-
4
1
-
-
2
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
3
-
4
1
-
-
-
-
-
-
-
-
-
6
1
1
-
3
3
1
1
-
-
-
-
-
-
3
3
727225
Choi
Characterization of a recombin ...
Dictyoglomus turgidum, Dictyoglomus turgidum DSM 6724
Biotechnol. Lett.
34
1079-1085
2012
-
-
1
-
-
-
2
4
-
2
2
2
-
2
-
-
1
-
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
4
-
2
2
2
-
-
-
1
-
-
11
-
16
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
713845
Patel
Substrate specificity of the B ...
Bacillus licheniformis
Appl. Environ. Microbiol.
77
3343-3350
2011
-
-
1
-
-
-
-
2
-
4
1
-
-
4
-
-
1
-
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
1
-
-
-
-
1
-
-
2
-
3
1
-
1
1
2
1
-
-
-
-
-
-
-
2
2
716872
Marles-Wright
The structure of a D-lyxose is ...
Bacillus subtilis 168, Bacillus subtilis
Proteins Struct. Funct. Bioinform.
79
2015-2019
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714568
Park
Mannose production from fructo ...
Providencia stuartii
Biotechnol. Lett.
32
1305-1309
2010
-
-
-
-
-
2
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
715675
Kwon
Substrate specificity of a rec ...
Providencia stuartii, Providencia stuartii KCTC 2568
J. Biosci. Bioeng.
110
26-31
2010
-
1
1
-
-
-
7
4
-
2
3
-
-
3
-
-
1
-
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
1
1
-
-
-
-
-
7
-
4
-
2
3
-
-
-
-
1
-
-
20
-
18
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
716074
Park
Substrate specificity of a rec ...
Serratia proteamaculans, Serratia proteamaculans KCTC 2936
Lett. Appl. Microbiol.
51
343-350
2010
-
-
1
-
-
-
5
4
-
2
3
-
-
3
-
-
1
-
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
2
3
-
-
-
-
1
-
-
10
-
13
1
1
-
1
4
1
-
-
-
-
-
-
-
4
4
674319
Cho
Characterization of a novel D- ...
Cohnella laeviribosi, Cohnella laeviribosi RI-39
J. Bacteriol.
189
1655-1663
2007
-
-
1
-
-
-
-
3
-
3
1
-
-
5
-
-
1
-
-
-
2
1
6
1
1
-
-
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
3
1
-
-
-
-
1
-
-
2
1
6
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
2647
Izumori
Pentose metabolism in Mycobact ...
Mycolicibacterium smegmatis
J. Bacteriol.
128
587-591
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2639
Anderson
-
D-Lyxose isomerase ...
Klebsiella aerogenes
Methods Enzymol.
9
593-596
1966
-
-
-
-
-
-
1
2
-
5
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
5
1
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2638
Anderson
Purification and characterizat ...
Klebsiella aerogenes
J. Biol. Chem.
240
2367-2372
1965
-
-
-
-
-
-
2
2
-
5
1
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
5
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-