Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.14 extracted from

  • Xu, W.; Zhang, W.; Tian, Y.; Zhang, T.; Jiang, B.; Mu, W.
    Characterization of a novel thermostable L-rhamnose isomerase from Thermobacillus composti KWC4 and its application for production of D-allose (2017), Process Biochem., 53, 153-161 .
No PubMed abstract available
Search for more literature for 5.3.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermobacillus composti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33.8
-
 L-rhamnose pH 7.5, 65°C Thermobacillus composti
47.9
-
 L-Mannose pH 7.5, 65°C Thermobacillus composti
70.4
-
 D-psicose pH 7.5, 65°C Thermobacillus composti
87.9
-
 D-allose pH 7.5, 65°C Thermobacillus composti

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 93% of the activity with Mn2+ Thermobacillus composti
Mg2+ 1 mM, 64% of the activity with Mn2+ Thermobacillus composti
Mn2+ 1 mM, best activator Thermobacillus composti
Ni2+ 1 mM, 78% of the activity with Mn2+ Thermobacillus composti

Organism

Organism UniProt Comment Textmining
Thermobacillus composti L0ECT7
-
-
Thermobacillus composti DSM 18247 L0ECT7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Thermobacillus composti D-psicose
-
 r
D-allose
-
 Thermobacillus composti DSM 18247 D-psicose
-
 r
D-psicose
-
 Thermobacillus composti D-allose
-
 r
D-psicose
-
 Thermobacillus composti DSM 18247 D-allose
-
 r
L-Mannose
-
 Thermobacillus composti L-Fructose
-
 r
L-Mannose
-
 Thermobacillus composti DSM 18247 L-Fructose
-
 r
L-Rhamnose
-
 Thermobacillus composti L-Rhamnulose
-
 r
L-Rhamnose
-
 Thermobacillus composti DSM 18247 L-Rhamnulose
-
 r

Subunits

Subunits Comment Organism
? x * 465850, calculated from sequence, x * 46000, SDS-PAGE Thermobacillus composti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermobacillus composti

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75
-
 80% of maximum activity Thermobacillus composti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermobacillus composti

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 more than 60% of maximum activity Thermobacillus composti
8
-
 more than 60% loss of activity above Thermobacillus composti

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
 D-allose pH 7.5, 65°C Thermobacillus composti
0.04
-
 D-psicose pH 7.5, 65°C Thermobacillus composti
0.27
-
 L-Mannose pH 7.5, 65°C Thermobacillus composti
0.59
-
 L-rhamnose pH 7.5, 65°C Thermobacillus composti