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Literature summary for 5.3.1.14 extracted from
- Characterization of a thermostable recombinant L-rhamnose isomerase from Caldicellulosiruptor obsidiansis OB47 and its application for the production of L-fructose and L-rhamnulose (2018), J. Sci. Food Agric., 98, 2184-2193 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 5% of the activity with Co2+ | Caldicellulosiruptor obsidiansis |  |
| Co2+ | best activator | Caldicellulosiruptor obsidiansis | |
| Fe2+ | 21% of the activity with Co2+ | Caldicellulosiruptor obsidiansis | |
| Mg2+ | 34% of the activity with Co2+ | Caldicellulosiruptor obsidiansis | |
| Mn2+ | 95% of the activity with Co2+ | Caldicellulosiruptor obsidiansis | |
| Ni2+ | 39% of the activity with Co2+ | Caldicellulosiruptor obsidiansis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | divalent cation required | Caldicellulosiruptor obsidiansis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor obsidiansis | D9TIY8 | - |
- |
| Caldicellulosiruptor obsidiansis OB47 | D9TIY8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.6 | - |
substrate L-fructose, pH 8.0, 85°C | Caldicellulosiruptor obsidiansis |
| 13.7 | - |
substrate D-allose, pH 8.0, 85°C | Caldicellulosiruptor obsidiansis |
| 57.9 | - |
substrate L-mannose, pH 8.0, 85°C | Caldicellulosiruptor obsidiansis |
| 277 | - |
substrate L-rhamnose, pH 8.0, 85°C | Caldicellulosiruptor obsidiansis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Caldicellulosiruptor obsidiansis | D-psicose | - |
r | |
| D-allose | - |
Caldicellulosiruptor obsidiansis OB47 | D-psicose | - |
r | |
| L-fructose | - |
Caldicellulosiruptor obsidiansis | L-mannose | - |
r | |
| L-fructose | - |
Caldicellulosiruptor obsidiansis OB47 | L-mannose | - |
r | |
| L-Mannose | - |
Caldicellulosiruptor obsidiansis | L-Fructose | - |
r | |
| L-Mannose | - |
Caldicellulosiruptor obsidiansis OB47 | L-Fructose | - |
r | |
| L-Rhamnose | - |
Caldicellulosiruptor obsidiansis | L-Rhamnulose | - |
r | |
| L-Rhamnose | - |
Caldicellulosiruptor obsidiansis OB47 | L-Rhamnulose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48300, claculated from sequence, 4 * 48000, SDS-PAGE | Caldicellulosiruptor obsidiansis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| COB47_0655 | - |
Caldicellulosiruptor obsidiansis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Caldicellulosiruptor obsidiansis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life 24.75 h, presence of Co2+ | Caldicellulosiruptor obsidiansis |
| 80 | - |
half-life 4.15 h, presence of Co2+ | Caldicellulosiruptor obsidiansis |
| 85 | - |
half-life 3.3 h, presence of Co2+ | Caldicellulosiruptor obsidiansis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Caldicellulosiruptor obsidiansis |
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