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Literature summary for 5.3.1.14 extracted from

  • Park, C.
    Characterization of a recombinant L-rhamnose isomerase from Bacillus subtilis and its application on production of L-lyxose and L-mannose (2014), Biotechnol. Bioprocess Eng., 19, 18-25 .
No PubMed abstract available
Search for more literature for 5.3.1.14

Application

Application Comment Organism
synthesis 40 g/l L-lyxose is produced from 100 g/l L-xylulose by the enzyme during 60 min, while 25 g/l L-mannose is produced from 100 g/l L-fructose in 80 min Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
53
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis
86
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
97
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1 mM, 25fold increase in activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
194000
-
 gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis ATCC 23857
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.47
-
 substrate L-allose, pH 8.0, 60°C Bacillus subtilis
0.92
-
 substrate L-mannose, pH 8.0, 60°C Bacillus subtilis
2.58
-
 substrate L-lyxose, pH 8.0, 60°C Bacillus subtilis
3.58
-
 substrate L-rhamnose, pH 8.0, 60°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Bacillus subtilis D-psicose
-
 r
D-allose
-
 Bacillus subtilis ATCC 23857 D-psicose
-
 r
D-gulose
-
 Bacillus subtilis D-sorbose
-
 r
D-gulose
-
 Bacillus subtilis ATCC 23857 D-sorbose
-
 r
L-lyxose
-
 Bacillus subtilis L-xylulose
-
 r
L-lyxose
-
 Bacillus subtilis ATCC 23857 L-xylulose
-
 r
L-Mannose
-
 Bacillus subtilis L-Fructose
-
 r
L-Mannose
-
 Bacillus subtilis ATCC 23857 L-Fructose
-
 r
L-Rhamnose
-
 Bacillus subtilis L-Rhamnulose
-
 r
L-Rhamnose
-
 Bacillus subtilis ATCC 23857 L-Rhamnulose
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 48600, SDS-PAGE, 4 * 49468, calculated from sequence Bacillus subtilis

Synonyms

Synonyms Comment Organism
RhaA
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 2 h, 53% residual activity Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
37
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis
53
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
153
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis
0.62
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
2.89
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis