Crystallization (Comment) | Organism |
---|---|
mutant enzyme H101N in complex with L-rhamnose and D-allose, vapor diffusion method, using 7-8% (w/v) polyethylene glycol 20000 and 50 mM MES buffer (pH 6.3) | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
D327N | inactive | Pseudomonas stutzeri |
H101N | the enzymatic activity of the mutant is decreased | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.92 | - |
L-rhamnose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
2.7 | - |
D-allose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
9.4 | - |
L-rhamnose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
60 | - |
D-allose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Rhamnose | Pseudomonas stutzeri | - |
L-Rhamnulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | Q75WH8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Pseudomonas stutzeri | D-psicose | - |
r | |
L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-RhI | - |
Pseudomonas stutzeri |
Rhamnose isomerase | - |
Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.8 | - |
D-allose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
24.5 | - |
D-allose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
192 | - |
L-rhamnose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
374 | - |
L-rhamnose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
D-allose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
6.2 | - |
D-allose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
20 | - |
L-rhamnose | mutant enzyme H101N, pH and temperature not specified in the publication | Pseudomonas stutzeri | |
410 | - |
L-rhamnose | wild type enzyme, pH and temperature not specified in the publication | Pseudomonas stutzeri |