Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.14 extracted from

  • Yoshida, H.; Takeda, K.; Izumori, K.; Kamitori, S.
    Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeri L-rhamnose isomerase (2010), Protein Eng. Des. Sel., 23, 919-927.
    View publication on PubMed
Search for more literature for 5.3.1.14

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzymes S329F, S329K, S329L, and S329A in complex with L-rhamnose, D-psicose or D-allose, droplet vapor diffusion method, using 6-9.25% (w/v) polyethylene glycol 20000, 50 mM MES buffer, pH 6.1-6.3, at 20°C Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
S329A mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
S329F mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
S329K mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri
S329L mutant showing reduced catalytic efficiency compared to the wild type enzyme Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.9
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
12.2
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
18.5
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
19.4
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
24.2
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
25.9
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
33.9
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
35.6
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
39.1
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
44.4
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
44.7
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
53.3
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
65.6
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
67.3
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
85.1
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri Q75WH8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allose
-
 Pseudomonas stutzeri D-psicose
-
 r
D-ribose
-
 Pseudomonas stutzeri D-ribulose
-
 r
L-Rhamnose
-
 Pseudomonas stutzeri L-Rhamnulose
-
 r
additional information the enzyme recognizes substrates at C2 and C3 but not at C4 and C5 Pseudomonas stutzeri ?
-
 ?

Synonyms

Synonyms Comment Organism
L-RhI
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
1.8
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
2.7
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
3.3
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
3.8
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
5.1
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
7.4
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
8.9
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
9.8
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
11.4
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
13.3
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
21.2
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
53.6
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
142
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
224
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0027
-
 D-ribose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.0043
-
 D-ribose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.005
-
 D-ribose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.071
-
 D-allose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.081
-
 D-allose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.13
-
 D-ribose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.2
-
 D-ribose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.27
-
 D-allose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.28
-
 D-allose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.41
-
 L-rhamnose mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.47
-
 D-allose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
0.55
-
 L-rhamnose mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
6.8
-
 L-rhamnose mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
7.7
-
 L-rhamnose mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri
11.6
-
 L-rhamnose wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C Pseudomonas stutzeri