Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37 | - |
L-rhamnose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
69 | - |
L-Lyxose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
76 | - |
L-Mannose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
111 | - |
L-xylulose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
123 | - |
L-Fructose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | enzyme activity is the highest with Mn2+, the optimal Mn2+ concentration is 1 mM (6.7fold increase activity relative to no treatment) | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45527 | - |
4 * 45527, calculated from amino acid sequence | Thermotoga maritima |
46000 | - |
4 * 46000, SDS-PAGE | Thermotoga maritima |
184000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima ATCC 43589 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
1.1 | - |
using D-psicose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
2.8 | - |
using D-ribose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
5.1 | - |
using L-fructose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
5.3 | - |
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
6.7 | - |
using D-allose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
6.8 | - |
using D-ribulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
8.6 | - |
using L-xylulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
15 | - |
using L-mannose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
20 | - |
using L-lyxose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
55 | - |
using L-rhamnose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allose | - |
Thermotoga maritima | D-psicose | - |
r | |
D-gulose | - |
Thermotoga maritima | D-sorbose | - |
r | |
D-ribose | - |
Thermotoga maritima | D-ribulose | - |
r | |
D-ribose | - |
Thermotoga maritima ATCC 43589 | D-ribulose | - |
r | |
L-fructose | - |
Thermotoga maritima | L-mannose | - |
r | |
L-lyxose | - |
Thermotoga maritima | L-xylulose | - |
r | |
L-lyxose | - |
Thermotoga maritima ATCC 43589 | L-xylulose | - |
r | |
L-Mannose | - |
Thermotoga maritima | L-Fructose | - |
r | |
L-Mannose | - |
Thermotoga maritima ATCC 43589 | L-Fructose | - |
r | |
L-Rhamnose | highest specific activity | Thermotoga maritima | L-Rhamnulose | - |
r | |
L-Rhamnose | highest specific activity | Thermotoga maritima ATCC 43589 | L-Rhamnulose | - |
r | |
L-xylulose | - |
Thermotoga maritima | L-lyxose | - |
r | |
additional information | no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate | Thermotoga maritima | ? | - |
? | |
additional information | no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate | Thermotoga maritima ATCC 43589 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, SDS-PAGE | Thermotoga maritima |
tetramer | 4 * 45527, calculated from amino acid sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
RhaA | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 95 | the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
146 | - |
L-rhamnose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
274 | - |
L-Fructose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
281 | - |
L-xylulose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
442 | - |
L-Mannose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
462 | - |
L-Lyxose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.23 | - |
L-Fructose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
2.55 | - |
L-xylulose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
5.82 | - |
L-Mannose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
6.72 | - |
L-Lyxose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima | |
39.36 | - |
L-rhamnose | purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0) | Thermotoga maritima |