BRENDA - Enzyme Database show
show all sequences of 5.3.1.14

Characterization of a recombinant thermostable L-rhamnose isomerase from Thermotoga maritima ATCC 43589 and its application in the production of L-lyxose and L-mannose

Park, C.S.; Yeom, S.J.; Lim, Y.R.; Kim, Y.S.; Oh, D.K.; Biotechnol. Lett. 32, 1947-1953 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Thermotoga maritima
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
37
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
69
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
76
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
111
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
123
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
enzyme activity is the highest with Mn2+, the optimal Mn2+ concentration is 1 mM (6.7fold increase activity relative to no treatment)
Thermotoga maritima
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45527
-
4 * 45527, calculated from amino acid sequence
Thermotoga maritima
46000
-
4 * 46000, SDS-PAGE
Thermotoga maritima
184000
-
gel filtration
Thermotoga maritima
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima ATCC 43589
-
-
-
Purification (Commentary)
Commentary
Organism
His-Trap column chromatography
Thermotoga maritima
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.5
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
1.1
-
using D-psicose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
2.8
-
using D-ribose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.1
-
using L-fructose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.3
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.7
-
using D-allose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.8
-
using D-ribulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
8.6
-
using L-xylulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
15
-
using L-mannose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
20
-
using L-lyxose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
55
-
using L-rhamnose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Allose
-
714573
Thermotoga maritima
D-Psicose
-
-
-
r
D-gulose
-
714573
Thermotoga maritima
D-sorbose
-
-
-
r
D-Ribose
-
714573
Thermotoga maritima
D-Ribulose
-
-
-
r
D-Ribose
-
714573
Thermotoga maritima ATCC 43589
D-Ribulose
-
-
-
r
L-fructose
-
714573
Thermotoga maritima
L-mannose
-
-
-
r
L-Lyxose
-
714573
Thermotoga maritima
L-Xylulose
-
-
-
r
L-Lyxose
-
714573
Thermotoga maritima ATCC 43589
L-Xylulose
-
-
-
r
L-Mannose
-
714573
Thermotoga maritima
L-Fructose
-
-
-
r
L-Mannose
-
714573
Thermotoga maritima ATCC 43589
L-Fructose
-
-
-
r
L-Rhamnose
highest specific activity
714573
Thermotoga maritima
L-Rhamnulose
-
-
-
r
L-Rhamnose
highest specific activity
714573
Thermotoga maritima ATCC 43589
L-Rhamnulose
-
-
-
r
L-xylulose
-
714573
Thermotoga maritima
L-lyxose
-
-
-
r
additional information
no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate
714573
Thermotoga maritima
?
-
-
-
-
additional information
no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate
714573
Thermotoga maritima ATCC 43589
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 45527, calculated from amino acid sequence; 4 * 46000, SDS-PAGE
Thermotoga maritima
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Thermotoga maritima
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
95
the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively
Thermotoga maritima
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
146
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
274
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
281
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
442
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
462
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
-
-
Thermotoga maritima
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli ER2566 cells
Thermotoga maritima
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
37
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
69
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
76
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
111
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
123
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
enzyme activity is the highest with Mn2+, the optimal Mn2+ concentration is 1 mM (6.7fold increase activity relative to no treatment)
Thermotoga maritima
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45527
-
4 * 45527, calculated from amino acid sequence
Thermotoga maritima
46000
-
4 * 46000, SDS-PAGE
Thermotoga maritima
184000
-
gel filtration
Thermotoga maritima
Purification (Commentary) (protein specific)
Commentary
Organism
His-Trap column chromatography
Thermotoga maritima
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.5
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
1.1
-
using D-psicose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
2.8
-
using D-ribose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.1
-
using L-fructose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.3
-
using D-gulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.7
-
using D-allose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.8
-
using D-ribulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
8.6
-
using L-xylulose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
15
-
using L-mannose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
20
-
using L-lyxose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
55
-
using L-rhamnose as substrate, purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-Allose
-
714573
Thermotoga maritima
D-Psicose
-
-
-
r
D-gulose
-
714573
Thermotoga maritima
D-sorbose
-
-
-
r
D-Ribose
-
714573
Thermotoga maritima
D-Ribulose
-
-
-
r
D-Ribose
-
714573
Thermotoga maritima ATCC 43589
D-Ribulose
-
-
-
r
L-fructose
-
714573
Thermotoga maritima
L-mannose
-
-
-
r
L-Lyxose
-
714573
Thermotoga maritima
L-Xylulose
-
-
-
r
L-Lyxose
-
714573
Thermotoga maritima ATCC 43589
L-Xylulose
-
-
-
r
L-Mannose
-
714573
Thermotoga maritima
L-Fructose
-
-
-
r
L-Mannose
-
714573
Thermotoga maritima ATCC 43589
L-Fructose
-
-
-
r
L-Rhamnose
highest specific activity
714573
Thermotoga maritima
L-Rhamnulose
-
-
-
r
L-Rhamnose
highest specific activity
714573
Thermotoga maritima ATCC 43589
L-Rhamnulose
-
-
-
r
L-xylulose
-
714573
Thermotoga maritima
L-lyxose
-
-
-
r
additional information
no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate
714573
Thermotoga maritima
?
-
-
-
-
additional information
no activity is observed with D-ribose 5-phosphate, D-glucose 6-phosphate and D-mannose 6-phosphate
714573
Thermotoga maritima ATCC 43589
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 45527, calculated from amino acid sequence; 4 * 46000, SDS-PAGE
Thermotoga maritima
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Thermotoga maritima
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
75
95
the half-lives of the enzyme at 75°C, 80°C, 85°C, 90°C and 95°C are 773 h, 347 h, 187 h, 118 h, and 65 h, respectively
Thermotoga maritima
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
146
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
274
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
281
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
442
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
462
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
-
-
Thermotoga maritima
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.23
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
2.55
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.82
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.72
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
39.36
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.23
-
L-Fructose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
2.55
-
L-xylulose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
5.82
-
L-Mannose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
6.72
-
L-Lyxose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
39.36
-
L-rhamnose
purified enzyme, at 85°C for 10 min in 50 mM EPPS buffer (pH 8.0)
Thermotoga maritima
Other publictions for EC 5.3.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747394
Seo
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-
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-
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1
1
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-
3
-
-
-
-
-
-
-
-
13
1
1
-
3
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
13
1
1
-
3
-
1
-
-
-
-
-
-
-
-
-
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6
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
4
-
8
1
1
-
3
-
1
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
8
1
1
-
3
-
1
-
-
-
-
-
-
-
-
-
749202
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53
153-161
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-
-
1
-
-
-
-
4
-
4
-
-
-
2
-
-
-
-
-
-
-
-
8
1
1
1
-
-
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
-
-
8
1
1
1
-
-
1
2
-
-
-
-
-
-
4
4
747820
Bai
-
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Bacillus subtilis, Bacillus subtilis 168
Food Sci. Technol. Res.
21
13-22
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-
-
1
-
-
-
8
4
-
3
-
-
-
2
-
-
-
-
-
-
-
-
10
1
1
-
1
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
4
-
3
-
-
-
-
-
-
-
-
-
-
10
1
1
-
1
4
-
-
-
-
-
-
-
-
4
4
747355
Park
-
Characterization of a recombi ...
Bacillus subtilis, Bacillus subtilis ATCC 23857
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19
18-25
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-
1
1
-
-
-
-
3
-
1
1
-
-
2
-
-
-
-
-
-
4
-
10
1
1
-
1
3
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
1
1
-
-
-
-
-
-
-
4
-
10
1
1
-
1
3
1
-
-
-
-
-
-
-
3
3
727230
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Biotechnol. Lett.
35
259-264
2013
-
-
1
-
-
-
-
7
-
1
3
2
-
3
-
-
1
-
-
-
-
-
12
1
1
-
1
7
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
-
1
3
2
-
-
-
1
-
-
-
-
12
1
1
-
1
7
1
-
1
-
-
-
-
-
7
7
727537
Yoshida
Structure of L-rhamnose isomer ...
Pseudomonas stutzeri
FEBS Open Bio
3
35-40
2013
-
-
-
1
2
-
-
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
713898
Prabhu
Cloning and characterization o ...
Bacillus halodurans, Bacillus halodurans ATCC BAA-125
Appl. Microbiol. Biotechnol.
89
635-644
2011
-
-
1
-
-
-
-
3
-
5
3
-
-
6
-
-
1
-
-
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