Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Mesorhizobium loti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | enzyme activity is lost after EDTA treatment | Mesorhizobium loti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.4 | L-Mannose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
5 | - |
L-rhamnose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
5.25 | - |
L-talose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
6.18 | - |
D-ribose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
7.11 | - |
D-allose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
23.5 | - |
L-Lyxose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highest activity in the presence of Mn2+ | Mesorhizobium loti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46946 | - |
x * 46946, calculated from amino acid sequence | Mesorhizobium loti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesorhizobium loti | - |
- |
- |
Mesorhizobium loti Tono | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mesorhizobium loti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Allose | - |
Mesorhizobium loti | ? | - |
? | |
D-Allose | - |
Mesorhizobium loti Tono | ? | - |
? | |
D-allose | - |
Mesorhizobium loti | D-talose | - |
? | |
D-Arabinose | slight activity | Mesorhizobium loti | ? | - |
? | |
D-Galactose | slight activity | Mesorhizobium loti | ? | - |
? | |
D-glucose | slight activity | Mesorhizobium loti | ? | - |
? | |
D-Lyxose | slight activity | Mesorhizobium loti | ? | - |
? | |
D-Mannose | - |
Mesorhizobium loti | ? | - |
? | |
D-Mannose | slight activity | Mesorhizobium loti | ? | - |
? | |
D-ribose | - |
Mesorhizobium loti | D-ribulose | - |
? | |
D-ribose | - |
Mesorhizobium loti Tono | D-ribulose | - |
? | |
D-Ribose | - |
Mesorhizobium loti | ? | - |
? | |
D-talose | slight activity | Mesorhizobium loti | D-allose | - |
? | |
D-talose | slight activity | Mesorhizobium loti | ? | - |
? | |
L-Arabinose | slight activity | Mesorhizobium loti | ? | - |
? | |
L-galactose | slight activity | Mesorhizobium loti | ? | - |
? | |
L-lyxose | - |
Mesorhizobium loti | L-xylulose | - |
r | |
L-lyxose | - |
Mesorhizobium loti Tono | L-xylulose | - |
r | |
L-lyxose | - |
Mesorhizobium loti | ? | - |
r | |
L-Mannose | - |
Mesorhizobium loti | L-Fructose | - |
? | |
L-Mannose | - |
Mesorhizobium loti Tono | L-Fructose | - |
? | |
L-mannose | - |
Mesorhizobium loti | ? | - |
? | |
L-Rhamnose | highest activity | Mesorhizobium loti | L-Rhamnulose | - |
? | |
L-Rhamnose | highest activity | Mesorhizobium loti Tono | L-Rhamnulose | - |
? | |
L-ribose | slight activity | Mesorhizobium loti | ? | - |
? | |
L-talose | - |
Mesorhizobium loti | ? | - |
? | |
L-xylulose | - |
Mesorhizobium loti | L-lyxose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 46946, calculated from amino acid sequence | Mesorhizobium loti |
Synonyms | Comment | Organism |
---|---|---|
L-RhI | - |
Mesorhizobium loti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mesorhizobium loti |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
after incubation at 50°C in glycine-NaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained | Mesorhizobium loti |
50 | - |
after incubation at 50°C in glycineNaOH buffer (pH 9.0) for 1 h, approximately 70% of enzyme activity is retained | Mesorhizobium loti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
D-ribose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
1.3 | - |
D-allose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
7 | - |
L-talose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
10 | - |
L-Mannose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
50.5 | - |
L-rhamnose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
73.8 | - |
L-Lyxose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mesorhizobium loti |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | the enzyme remains stable after 24 h over a broad pH range from pH 5.0-11.0 | Mesorhizobium loti |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-allose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
0.4 | - |
L-Mannose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
0.8 | - |
D-ribose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
1.3 | - |
L-talose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
3.1 | - |
L-Lyxose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti | |
10.1 | - |
L-rhamnose | in 50 mM glycine-NaOH, pH 9.0, 1 mM MnCl2, at 60°C | Mesorhizobium loti |