Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Halalkalibacterium halodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
119 | - |
L-Mannose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans | |
528 | - |
L-rhamnose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans | |
771 | - |
L-Lyxose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | required for catalytic activity, 93% activity at 1 mM compared to Mn2+ | Halalkalibacterium halodurans | |
Mg2+ | required for catalytic activity, 57% activity at 1 mM compared to Mn2+ | Halalkalibacterium halodurans | |
Mn2+ | required for catalytic activity, 100% activity at 1 mM | Halalkalibacterium halodurans | |
additional information | enzyme without any metal ions shows a negligible activity. The enzyme activity is not stimulated by Ca2+, Ba2+, Na2+, Fe2+, or Cu2+ | Halalkalibacterium halodurans | |
Zn2+ | required for catalytic activity, 20% activity at 1 mM compared to Mn2+ | Halalkalibacterium halodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, SDS-PAGE | Halalkalibacterium halodurans |
48178 | - |
2 * 48178, calculated from amino acid sequence | Halalkalibacterium halodurans |
121000 | - |
gel filtration | Halalkalibacterium halodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
- |
- |
Halalkalibacterium halodurans ATCC BAA-125 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-gulose | 5% activity compared to L-rhamnose | Halalkalibacterium halodurans | D-sorbose | - |
? | |
D-gulose | 5% activity compared to L-rhamnose | Halalkalibacterium halodurans ATCC BAA-125 | D-sorbose | - |
? | |
L-lyxose | 144% activity compared to L-rhamnose | Halalkalibacterium halodurans | L-xylulose | - |
? | |
L-lyxose | 144% activity compared to L-rhamnose | Halalkalibacterium halodurans ATCC BAA-125 | L-xylulose | - |
? | |
L-Mannose | 59% activity compared to L-rhamnose | Halalkalibacterium halodurans | L-Fructose | - |
? | |
L-Mannose | 59% activity compared to L-rhamnose | Halalkalibacterium halodurans ATCC BAA-125 | L-Fructose | - |
? | |
L-Rhamnose | 100% activity | Halalkalibacterium halodurans | L-Rhamnulose | - |
r | |
L-Rhamnose | 100% activity | Halalkalibacterium halodurans ATCC BAA-125 | L-Rhamnulose | - |
r | |
L-talose | 3% activity compared to L-rhamnose | Halalkalibacterium halodurans | ? | - |
? | |
L-talose | 3% activity compared to L-rhamnose | Halalkalibacterium halodurans ATCC BAA-125 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48000, SDS-PAGE | Halalkalibacterium halodurans |
homodimer | 2 * 48178, calculated from amino acid sequence | Halalkalibacterium halodurans |
Synonyms | Comment | Organism |
---|---|---|
L-RhI | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Halalkalibacterium halodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme shows 100% activity for 10 h at 60°C, the half-life of the enzyme is more than 900 min, about 25 min, and 5 min at 60°C, 70°C, and 80°C, respectively | Halalkalibacterium halodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
88.88 | - |
L-Mannose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans | |
149.5 | - |
L-rhamnose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans | |
216 | - |
L-Lyxose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Halalkalibacterium halodurans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the optimum pH for isomerization is pH 7.0, with 75%, 85%, 92%, and 90% of maximum activity at pH 6.0, 8.0, 9.0 and 10.0, respectively | Halalkalibacterium halodurans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Halalkalibacterium halodurans | isoelectric focusing | - |
5.5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.283 | - |
L-rhamnose | in 50 mM malate buffer, at pH 7.0 and 70°C | Halalkalibacterium halodurans |