Literature summary for 5.3.1.14 extracted from

Doan, T.N.; Prabhu, P.; Kim, J.K.; Ahn, Y.J.; Natarajan, S.; Kang, L.W.; Park, G.T.; Lim, S.B.; Lee, J.K.
Crystallization and preliminary X-ray crystallographic analysis of L-rhamnose isomerase with a novel high thermostability from Bacillus halodurans (2010), Acta Crystallogr. Sect. F, 66, 677-680.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Halalkalibacterium halodurans

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 10% (w/v) PEG 8000, 0.1 M HEPES pH 6.0 and 0.2 M sodium acetate	Halalkalibacterium halodurans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48000	-	x * 48000, SDS-PAGE	Halalkalibacterium halodurans

Organism

Organism	UniProt	Comment	Textmining
Halalkalibacterium halodurans	-	-	-
Halalkalibacterium halodurans ATCC BAA-125	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Rhamnose	i.e 6-deoxy-L-mannose	Halalkalibacterium halodurans	L-Rhamnulose	i.e 6-deoxy-L-fructose	r
L-Rhamnose	i.e 6-deoxy-L-mannose	Halalkalibacterium halodurans ATCC BAA-125	L-Rhamnulose	i.e 6-deoxy-L-fructose	r

Subunits

Subunits	Comment	Organism
?	x * 48000, SDS-PAGE	Halalkalibacterium halodurans

Synonyms

Synonyms	Comment	Organism
L-RhI	-	Halalkalibacterium halodurans

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Release 2023.1 (January 2023)