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Literature summary for 5.3.1.14 extracted from
- Efficient biosynthesis of D-allose from D-psicose by cross-linked recombinant L-rhamnose isomerase: separation of product by ethanol crystallization (2006), J. Biosci. Bioeng., 101, 340-345.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme, cross-linked with glutaraldehyde and L-lysine, is used to produce D-allose from D-piscose, which is derived from D-fructose by a recombinant D-tagatose 3-epimerase, in a bioreactor plan, method optimization, overview | Pseudomonas stutzeri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | cross-linking of the enzyme with glutaraldehyde and L-lysine, for usage in production of D-allose from D-piscose, overview | Pseudomonas stutzeri |
General Stability
| General Stability | Organism |
|---|---|
| the cross-linked enzyme shows a catalytic half-life of 2 months | Pseudomonas stutzeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-psicose | Pseudomonas stutzeri | - |
D-allose | - |
? |  |
| L-Rhamnose | Pseudomonas stutzeri | - |
L-Rhamnulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-psicose | - |
Pseudomonas stutzeri | D-allose | - |
? | |
| D-psicose | - |
Pseudomonas stutzeri | D-allose | the product mixture contains 25% D-allose, 8% D-altrose, and 67% D-psicose | ? | |
| L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
free enzyme | Pseudomonas stutzeri |
| 60 | - |
cross-linked enzyme | Pseudomonas stutzeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
up to, 10 min, cross-linked enzyme, stable | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas stutzeri |
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