Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli | Pseudomonas stutzeri |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged L-RhI by hanging-drop vapour-diffusion method, 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, is mixed with 0.002 ml reservcoir solution containing 7-8% w/v PEG 20 000 and 50 mM MES buffer, pH 6.3, equilibration against 0.5 ml reservoir solution, X-ray diffraction structure determination and analysis at 2.0 A resolution | Pseudomonas stutzeri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
47681 | - |
x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Rhamnose | Pseudomonas stutzeri | - |
L-Rhamnulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | Q75WH8 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r | |
L-Rhamnose | L-RhI catalyzes not only the reversible isomerization of L-rhamnose to L-rhamnulose, but also isomerization between various rare aldoses and ketoses | Pseudomonas stutzeri | L-Rhamnulose | - |
r | |
additional information | the Pseudomonas stutzeri enzyme shows a broader substrate specificity compared to the enzyme from Escherichia coli, overview | Pseudomonas stutzeri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE, x * 47681, recombinant His-tagged enzyme, MALDI-TOF mass spectrometry | Pseudomonas stutzeri |
Synonyms | Comment | Organism |
---|---|---|
L-rhamnose isomerase | - |
Pseudomonas stutzeri |
L-RhI | - |
Pseudomonas stutzeri |