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Literature summary for 5.3.1.14 extracted from
- Cloning, nucleotide sequence, and overexpression of the L-rhamnose isomerase gene from Pseudomonas stutzeri in Escherichia coli (2004), Appl. Environ. Microbiol., 70, 3298-3304.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM 109 | Pseudomonas stutzeri |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.9 | - |
L-rhamnose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri |  |
| 38.5 | - |
D-ribose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 42 | - |
D-allose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 55 | - |
L-rhamnose | 50°C, pH 9.0, intrinsic enzyme from Pseudomonas stutzeri | Pseudomonas stutzeri | |
| 55.5 | - |
L-Mannose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 61.7 | - |
L-Lyxose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 71 | - |
D-altrose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 127.4 | - |
D-arabinose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 203 | - |
L-Xylose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 250 | - |
D-xylose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
| 564 | - |
D-glucose | 50°C, pH 9.0, recombinant enzyme | Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, SDS-PAGE | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | Q75WH8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140 | - |
- |
Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Allose | - |
Pseudomonas stutzeri | ? | - |
? | |
| D-altrose | - |
Pseudomonas stutzeri | ? | - |
? | |
| D-Arabinose | - |
Pseudomonas stutzeri | ? | - |
? | |
| D-glucose | - |
Pseudomonas stutzeri | ? | - |
? | |
| D-Ribose | - |
Pseudomonas stutzeri | ? | - |
? | |
| D-Xylose | - |
Pseudomonas stutzeri | ? | - |
? | |
| L-lyxose | - |
Pseudomonas stutzeri | ? | - |
? | |
| L-mannose | - |
Pseudomonas stutzeri | ? | - |
? | |
| L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
? | |
| L-xylose | - |
Pseudomonas stutzeri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Pseudomonas stutzeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pseudomonas stutzeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable below | Pseudomonas stutzeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Pseudomonas stutzeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | stable | Pseudomonas stutzeri |
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