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Literature summary for 5.3.1.13 extracted from
- Identification of a D-arabinose-5-phosphate isomerase in the Gram-positive Clostridium tetani (2017), J. Bacteriol., 199, e00246 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridium tetani |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.89 | - |
D-arabinose 5-phosphate | pH 6.5, 37°C | Clostridium tetani |  |
| 6.65 | - |
D-ribulose 5-phosphate | pH 6.5, 37°C | Clostridium tetani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 109200 | - |
gel filtration | Clostridium tetani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium tetani | A0A4Q0VE60 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arabinose 5-phosphate | - |
Clostridium tetani | D-Ribulose 5-phosphate | - |
? | |
| D-ribulose 5-phosphate | - |
Clostridium tetani | D-arabinose 5-phosphate | - |
? | |
| additional information | no substrates: D-arabinose, D-arabinose-5-phosphate, D-glucose-6-phosphate, D-mannose-6-phosphate, D-glucosamine-6-phosphate, D-glucose-1-phosphate, and D-ribose-5-phosphate | Clostridium tetani | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 26000, SDS-PAGE, His-tagged recombinant protein, x * 25740, calculated from sequence | Clostridium tetani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| API | - |
Clostridium tetani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 86.7 | - |
D-ribulose 5-phosphate | pH 6.5, 37°C | Clostridium tetani | |
| 102 | - |
D-arabinose 5-phosphate | pH 6.5, 37°C | Clostridium tetani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | broad | Clostridium tetani |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | expression complements the API deficiency of an Escherichia coli API knockout strain | Clostridium tetani |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
D-ribulose 5-phosphate | pH 6.5, 37°C | Clostridium tetani | |
| 54 | - |
D-arabinose 5-phosphate | pH 6.5, 37°C | Clostridium tetani | |
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Release 2023.1 (January 2023)
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