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Literature summary for 5.3.1.13 extracted from

  • Airoldi, C.; Sommaruga, S.; Merlo, S.; Sperandeo, P.; Cipolla, L.; Polissi, A.; Nicotra, F.
    Targeting bacterial membranes: identification of Pseudomonas aeruginosa D-arabinose-5P isomerase and NMR characterisation of its substrate recognition and binding properties (2011), ChemBioChem, 12, 719-727.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
H190A inactive Pseudomonas aeruginosa
H85A inactive Pseudomonas aeruginosa
K56A the mutant shows 0.31% residual activity compared to the wild type enzyme Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Pseudomonas aeruginosa
34067
-
x * 34067, calculated from amino acid sequence Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Arabinose 5-phosphate Pseudomonas aeruginosa
-
D-Ribulose 5-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.092
-
mutant enzyme K56A, in Tris·HCl, (pH 8.5, 50 mM), at 37°C Pseudomonas aeruginosa
29.5
-
recombinant wild type enzyme, in Tris·HCl, (pH 8.5, 50 mM), at 37°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-D-threo-pentofuranose 5-phosphate the catalytic efficiency is about 1000times lower than for the natural substrate D-arabinose 5-phosphate Pseudomonas aeruginosa 3-deoxy-D-glycero-pentulose 5-phosphate
-
?
4-deoxy-D-threo-pentofuranose 5-phosphate the catalytic efficiency is comparable with the natural substrate D-arabinose 5-phosphate Pseudomonas aeruginosa 4-deoxy-D-glycero-pentulose 5-phosphate
-
?
D-Arabinose 5-phosphate
-
Pseudomonas aeruginosa D-Ribulose 5-phosphate
-
r
additional information no activity with D-ribose 5-phosphate Pseudomonas aeruginosa ?
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Pseudomonas aeruginosa
? x * 34067, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
API
-
Pseudomonas aeruginosa
D-arabinose-5P isomerase
-
Pseudomonas aeruginosa
KdsD
-
Pseudomonas aeruginosa

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthesis of 3-deoxy-D-manno-oct-2-ulosonic acid and thus of lipopolysaccharide Pseudomonas aeruginosa