Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | EDTA treatment increases the activity of c3406 protein by approximately 2.5fold | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Escherichia coli | |
Hg2+ | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
1.92 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | stimulates activity | Escherichia coli | |
Ca2+ | stimulates activity | Escherichia coli | |
Co2+ | stimulates activity | Escherichia coli | |
Cu2+ | stimulates activity | Escherichia coli | |
Fe2+ | stimulates activity | Escherichia coli | |
Mg2+ | stimulates activity | Escherichia coli | |
Mn2+ | stimulates activity | Escherichia coli | |
Ni2+ | stimulates activity | Escherichia coli | |
Zn2+ | c3406 protein possesses 0.72 equivalent of Zn2+ per subunit | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20888 | - |
x * 20888, mass spectrometry | Escherichia coli |
21000 | - |
x * 21000, SDS-PAGE | Escherichia coli |
73000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli CFT073 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and MonoQ column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
r | |
D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | - |
r | |
D-ribulose 5-phosphate | - |
Escherichia coli | D-arabinose 5-phosphate | - |
r | |
D-ribulose 5-phosphate | - |
Escherichia coli CFT073 | D-arabinose 5-phosphate | - |
r | |
additional information | D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates | Escherichia coli | ? | - |
? | |
additional information | D-ribose 5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose 6-phosphate, and D-glucosamine 6-phosphate are no substrates | Escherichia coli CFT073 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer or tetramer | x * 21000, SDS-PAGE | Escherichia coli |
trimer or tetramer | x * 20888, mass spectrometry | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
A5P isomerase | - |
Escherichia coli |
arabinose 5-phosphate isomerase | - |
Escherichia coli |
c3406 protein | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
16.8 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | only trace A5P isomerase activity observed below pH 5.5 or above pH 7.5 | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a c3406 deletion mutant is still able to compete with wild type CFT073 in a transurethral cochallenge in mice and cannot colonize the mouse kidney | Escherichia coli |
physiological function | the presence of c3406 is not essential for a pathogenic phenotype | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
D-arabinose 5-phosphate | pH 6.6, 37°C | Escherichia coli | |
15 | - |
D-ribulose 5-phosphate | pH 6.6, 37°C | Escherichia coli |