Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | Zn2+ inhibits substrate binding in a reversible manner | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Arabinose 5-phosphate | Escherichia coli | - |
D-Ribulose 5-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P45395 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-threo-pentofuranose 5-phosphate | the catalysis proceeds 10fold slower than for the natural substrate isomerization | Escherichia coli | 3-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
4-deoxy-D-threo-arabinose 5-phosphate | the isomerization rate for this compound is 2fold higher than that of the natural substrate | Escherichia coli | 4-deoxy-D-glycero-pentulose 5-phosphate | - |
? | |
D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
r | |
additional information | the enzyme does not isomerize D-xylulose-5-phosphate, D-arabinose, D-ribose, and D-ribulose | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
API | - |
Escherichia coli |
D-arabinose-5-phosphate isomerase | - |
Escherichia coli |
KdsD | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | API is a key enzyme in the biosynthesis of lipopolysaccharide, it catalyzes the first reaction of 3-deoxy-d-manno-octulosonate biosynthesis | Escherichia coli |