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Literature summary for 5.3.1.13 extracted from

  • Yep, A.; Sorenson, R.J.; Wilson, M.R.; Showalter, H.D.; Larsen, S.D.; Keller, P.R.; Woodard, R.W.
    Enediol mimics as inhibitors of the D-arabinose 5-phosphate isomerase (KdsD) from Francisella tularensis (2011), Bioorg. Med. Chem. Lett., 21, 2679-2682.
    View publication on PubMed
Search for more literature for 5.3.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Francisella tularensis
expressed in Escherichia coli BL21(DE3) cells Francisella tularensis

Inhibitors

Inhibitors Comment Organism Structure
(2R,3R)-2,3-dihydroxy-4-(hydroxyamino)-4-oxobutyl phosphate
-
 Francisella tularensis
(2R,3R)-4-amino-2,3-dihydroxy-4-oxobutyl phosphate
-
 Francisella tularensis
(2S,3R)-2,3-bis(acetyloxy)-4-(hydroxyamino)-4-oxobutyl phosphate
-
 Francisella tularensis
oxido(dioxo)[(2R,3R)-N,2,3-trihydroxy-4-(hydroxy-kO)butanamidato]phosphate(2-)
-
 Francisella tularensis
[(2R,3R)-1-(hydroxy-kO)-4-(hydroxyamino)-4-oxobutane-2,3-diyl diacetatato](oxido)dioxophosphate(2-)
-
 Francisella tularensis
[(2R,3R)-2,3-dihydroxy-4-(hydroxy-kO)butanamidato](oxido)dioxophosphate(2-)
-
 Francisella tularensis
[(4R,5R)-5-(hydroxycarbamoyl)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl phosphate
-
 Francisella tularensis
[(4R,5R)-N-hydroxy-5-[(hydroxy-kO)methyl]-2,2-dimethyl-1,3-dioxolane-4-carboxamidato](oxido)dioxophosphate(2-)
-
 Francisella tularensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 D-arabinose 5-phosphate at 37°C, pH not specified in the publication Francisella tularensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Arabinose 5-phosphate Francisella tularensis
-
 D-Ribulose 5-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Francisella tularensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Francisella tularensis
metal-affinity column chromatography Francisella tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Arabinose 5-phosphate
-
 Francisella tularensis D-Ribulose 5-phosphate
-
 r

Synonyms

Synonyms Comment Organism
A5PI
-
 Francisella tularensis
D-Arabinose 5-phosphate isomerase
-
 Francisella tularensis
KdsD
-
 Francisella tularensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
141
-
 D-arabinose 5-phosphate at 37°C, pH not specified in the publication Francisella tularensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 [(2R,3R)-1-(hydroxy-kO)-4-(hydroxyamino)-4-oxobutane-2,3-diyl diacetatato](oxido)dioxophosphate(2-) at 37°C, pH not specified in the publication Francisella tularensis
0.01
-
 [(2R,3R)-2,3-dihydroxy-4-(hydroxy-kO)butanamidato](oxido)dioxophosphate(2-) at 37°C, pH not specified in the publication Francisella tularensis
0.035
-
 oxido(dioxo)[(2R,3R)-N,2,3-trihydroxy-4-(hydroxy-kO)butanamidato]phosphate(2-) at 37°C, pH not specified in the publication Francisella tularensis
3
-
 [(4R,5R)-N-hydroxy-5-[(hydroxy-kO)methyl]-2,2-dimethyl-1,3-dioxolane-4-carboxamidato](oxido)dioxophosphate(2-) at 37°C, pH not specified in the publication Francisella tularensis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.007
-
 pH and temperature not specified in the publication Francisella tularensis (2S,3R)-2,3-bis(acetyloxy)-4-(hydroxyamino)-4-oxobutyl phosphate
0.01
-
 pH and temperature not specified in the publication Francisella tularensis (2R,3R)-4-amino-2,3-dihydroxy-4-oxobutyl phosphate
0.035
-
 pH and temperature not specified in the publication Francisella tularensis (2R,3R)-2,3-dihydroxy-4-(hydroxyamino)-4-oxobutyl phosphate
3
-
 pH and temperature not specified in the publication Francisella tularensis [(4R,5R)-5-(hydroxycarbamoyl)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl phosphate