Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-arabinaric acid | competitive inhibitor | Escherichia coli | |
D-arabinohydroxamate | competitive inhibitor | Escherichia coli | |
L-Gulonic acid | competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-glucuronate | reconstituted with Co2+, 30°C, pH 8 | Escherichia coli | |
0.48 | - |
D-glucuronate | reconstituted with Mn2+, 30°C, pH 8 | Escherichia coli | |
0.49 | - |
D-glucuronate | apoenzyme | Escherichia coli | |
0.5 | - |
D-glucuronate | reconstituted with Zn2+, 30°C, pH 8 | Escherichia coli | |
0.55 | - |
D-glucuronate | reconstituted with Cd2+, 30°C, pH 8 | Escherichia coli | |
8.5 | - |
D-glucuronate | reconstituted with Ni2+, 30°C, pH 8 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | enzyme contains 1 equiv of zinc per subunit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A8G3 | - |
- |
Purification (Comment) | Organism |
---|---|
of the wild type and recombinant protein, ammonium sulfate fractionation, gel filtration, and ion exchange chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-Glucuronate = D-fructuronate | reaction mechanisms in which an active site base abstracts the proton from C2 of D-glucuronate to form a cisenediol intermediate. The conjugate acid then transfers this proton to C1 of the cis-enediol intermediate to form D-fructuronate | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucuronate | - |
Escherichia coli | D-Fructuronate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Glucuronate isomerase | - |
Escherichia coli |
Uronate isomerase | URI | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
D-arabinaric acid | apo and Zn2+ reconstituted enzyme | Escherichia coli | |
0.00043 | - |
L-Gulonic acid | Zn2+ reconstituted enzyme | Escherichia coli | |
0.00067 | - |
D-arabinohydroxamate | Zn2+ reconstituted enzyme | Escherichia coli | |
0.00093 | - |
D-arabinohydroxamate | apo enzyme | Escherichia coli |