Literature summary for 5.3.1.1 extracted from

Garcia-Torres, I.; Cabrera, N.; Torres-Larios, A.; Rodriguez-Bolanos, M.; Diaz-Mazariegos, S.; Gomez-Puyou, A.; Perez-Montfort, R.
Identification of amino acids that account for long-range interactions in two triosephosphate isomerases from pathogenic trypanosomes (2011), PLoS ONE, 6, e18791.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli (DE3)pLysS cells	Trypanosoma brucei
expressed in Escherichia coli (DE3)pLysS cells	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
4,4'-Dithiopyridine	-	Trypanosoma brucei
4,4'-Dithiopyridine	-	Trypanosoma cruzi
5,5'-dithiobis(2-nitrobenzoate)	-	Trypanosoma brucei
5,5'-dithiobis(2-nitrobenzoate)	-	Trypanosoma cruzi
methylmethane thiosulfonate	60% residual activity at 0.1 mM	Trypanosoma brucei
methylmethane thiosulfonate	almost complete inhibition at 0.1 mM	Trypanosoma cruzi
N-ethylmaleimide	-	Trypanosoma brucei
N-ethylmaleimide	-	Trypanosoma cruzi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma cruzi
0.45	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	Trypanosoma brucei	-	Glycerone phosphate	-	r
D-Glyceraldehyde 3-phosphate	Trypanosoma cruzi	-	Glycerone phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	-	-	-
Trypanosoma cruzi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography	Trypanosoma brucei
ammonium sulfate precipitation, Toyopearl column chromatography and SP-Sepharose column chromatography	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	-	Trypanosoma brucei	Glycerone phosphate	-	r
D-Glyceraldehyde 3-phosphate	-	Trypanosoma cruzi	Glycerone phosphate	-	r

Synonyms

Synonyms	Comment	Organism
TIM	-	Trypanosoma brucei
TIM	-	Trypanosoma cruzi
Triosephosphate isomerase	-	Trypanosoma brucei
Triosephosphate isomerase	-	Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4500	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma cruzi
5167	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10500	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma cruzi
11500	-	D-glyceraldehyde 3-phosphate	in 100 mM TEA, 10 mM EDTA, pH 7.4, at 25°C	Trypanosoma brucei

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Release 2023.1 (January 2023)