Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphite | - |
Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
L232A | the mutation in a 6fold decrease in kcat/Km for the reversible isomerization of D-glyceraldehyde 3-phosphate to give dihydroxyacetone phosphate | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
dihydroxyacetone phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
0.14 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
0.25 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
0.7 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | Trypanosoma brucei brucei | - |
dihydroxyacetone phosphate | - |
r | |
dihydroxyacetone phosphate | Trypanosoma brucei brucei | - |
D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei brucei | dihydroxyacetone phosphate | - |
r | |
dihydroxyacetone phosphate | - |
Trypanosoma brucei brucei | D-glyceraldehyde 3-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Trypanosoma brucei brucei |
Triosephosphate isomerase | - |
Trypanosoma brucei brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
dihydroxyacetone phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
220 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
300 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
2100 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61 | - |
dihydroxyacetone phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
430 | - |
dihydroxyacetone phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
1500 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L232A, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei | |
8400 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, at pH 7.5 (30 mM triethanolamine), 25°C | Trypanosoma brucei brucei |