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Literature summary for 5.3.1.1 extracted from
- Tetrameric triosephosphate isomerase from hyperthermophilic Archaea (1996), FEBS Lett., 383, 245-250.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pyrococcus woesei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
4 * 24000, SDS-PAGE | Pyrococcus woesei |
| 27800 | - |
4 * 27800, SDS-PAGE | Methanothermus fervidus |
| 57000 | - |
gel filtration | Methanobacterium bryantii |
| 99600 | - |
gel filtration | Pyrococcus woesei |
| 100000 | - |
gel filtration, sucrose density ultracentrifugation | Methanothermus fervidus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanobacterium bryantii | O74025 | - |
- |
| Methanothermus fervidus | - |
- |
- |
| Methanothermus fervidus DSM 2088 | - |
- |
- |
| Pyrococcus woesei | P62003 | - |
- |
| Pyrococcus woesei DSM 3773 | P62003 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanothermus fervidus |
- |
Pyrococcus woesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate | - |
Methanothermus fervidus | D-glyceraldehyde 3-phosphate | - |
? |  |
| glycerone phosphate | - |
Pyrococcus woesei | D-glyceraldehyde 3-phosphate | - |
? | |
| glycerone phosphate | - |
Methanobacterium bryantii | D-glyceraldehyde 3-phosphate | - |
? | |
| glycerone phosphate | - |
Methanothermus fervidus DSM 2088 | D-glyceraldehyde 3-phosphate | - |
? | |
| glycerone phosphate | - |
Pyrococcus woesei DSM 3773 | D-glyceraldehyde 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Methanobacterium bryantii |
| homotetramer | 4 * 24000, SDS-PAGE | Pyrococcus woesei |
| homotetramer | 4 * 27800, SDS-PAGE | Methanothermus fervidus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TIM | - |
Methanothermus fervidus |
| TIM | - |
Pyrococcus woesei |
| TIM | - |
Methanobacterium bryantii |
| Triosephosphate isomerase | - |
Methanothermus fervidus |
| Triosephosphate isomerase | - |
Pyrococcus woesei |
| Triosephosphate isomerase | - |
Methanobacterium bryantii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
assay at | Methanothermus fervidus |
| 70 | - |
assay at | Pyrococcus woesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 104 | - |
half-life: 280 min | Pyrococcus woesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Methanothermus fervidus |
| 7.4 | - |
assay at | Pyrococcus woesei |
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Release 2023.1 (January 2023)
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