Crystallization (Comment) | Organism |
---|---|
monomeric enzyme variant with an engineered binding groove, m18bTIM, and the V233A mutant of this variant in complex with citrate, 2-phosphoglycolate, and glycerol 3-phosphate, to 1.89, 1.6, 2.3 and 2.0 A resolution, respectively | Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of mutation V233A into a monomeric enzyme variant with an engineered binding groove, m18bTIM. Mutation V233A restores the structural properties of loop-7, the binding site of a conserved water molecule between loop-7 and loop-8 and the binding site of the phosphate moiety of wild-type in the m18bTIM background. The active site of the V233A mutant can bind transition state analogs and suicide inhibitors competently, the catalytic efficiency of the V233A mutant is too low to be detected | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
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Trypanosoma brucei | P04789 | - |
- |