Protein Variants | Comment | Organism |
---|---|---|
K17A/Y46A/D48F/Q82A/D85S | mutation of residues in the dimer interface of enzyme | Saccharomyces cerevisiae |
K17L/Y46F/D48F/Q82F/D85L | mutation of residues in the dimer interface of enzyme. Decrease in catalytic efficiency by 4 orders of magnitude | Saccharomyces cerevisiae |
K17L/Y46F/D48Y/Q82A/D85A | mutation of residues in the dimer interface of enzyme. Decrease in catalytic efficiency by 4 orders of magnitude | Saccharomyces cerevisiae |
K17P/Y46A/D48L/Q82T/D85A | mutation of residues in the dimer interface of enzyme | Saccharomyces cerevisiae |
additional information | mutation of five residues in the dimer interface of enzyme. Obtained proteins are soluble, dimeric, and compact. Proteins obtained from direct evolution experiments show wild-type-like catalytic activity, while their stability is decreased. In silico-designed proteins are very stable dimers that bind substrate with a wild-type-like Km value, albeit they exhibit a very low kcat | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.09 | - |
D-glyceraldehyde 3-phosphate | mutant K17A/Y46A/D48F/Q82A/D85S , pH 7.4, 25°C | Saccharomyces cerevisiae | |
1.2 | - |
D-glyceraldehyde 3-phosphate | mutant K17L/Y46F/D48F/Q82F/D85L, pH 7.4, 25°C | Saccharomyces cerevisiae | |
1.26 | - |
D-glyceraldehyde 3-phosphate | mutant K17L/Y46F/D48Y/Q82A/D85A, pH 7.4, 25°C | Saccharomyces cerevisiae | |
1.4 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Saccharomyces cerevisiae | |
2.44 | - |
D-glyceraldehyde 3-phosphate | mutant K17P/Y46A/D48L/Q82T/D85A, pH 7.4, 25°C | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00942 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | dihydroxyacetone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
D-glyceraldehyde 3-phosphate | mutant K17L/Y46F/D48F/Q82F/D85L, pH 7.4, 25°C | Saccharomyces cerevisiae | |
1.04 | - |
D-glyceraldehyde 3-phosphate | mutant K17L/Y46F/D48Y/Q82A/D85A, pH 7.4, 25°C | Saccharomyces cerevisiae | |
4160 | - |
D-glyceraldehyde 3-phosphate | mutant K17P/Y46A/D48L/Q82T/D85A, pH 7.4, 25°C | Saccharomyces cerevisiae | |
6400 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Saccharomyces cerevisiae | |
7020 | - |
D-glyceraldehyde 3-phosphate | mutant K17A/Y46A/D48F/Q82A/D85S , pH 7.4, 25°C | Saccharomyces cerevisiae |