Protein Variants | Comment | Organism |
---|---|---|
C15A | dimer formation of mutant protein with intact Trypanosoma brucei monomer, maximal inhibition of catalysis by mutation is 60%. Dimer formation with catalytically inert Trypanosoma brucei mutant E168D causes a drop in activity by 50% | Trypanosoma cruzi |
E168D | catalytically inert. Dimer formation with Trypanosoma cruzi dimer interface mutant C15Acauses a drop in activity by 50% | Trypanosoma brucei |
additional information | hybrids of Trypanosoma cruzi enzyme carrying residues of the dimer interface from Trypanomsoma brucei and vice versa, and hybrids with one monomer in the enzyme dimer from Trypanosoma cruzi, and the second monomer from Trypanosoma brucei. Solvent exposure of the interfacial C15 depends predominantly on the characteristics of the adjoining monomer. Half of the activity of each monomer depends on the integrity of each of the two C15-loop3 portions of the interface | Trypanosoma brucei |
additional information | hybrids of Trypanosoma cruzi enzyme carrying residues of the dimer interface from Trypanomsoma brucei and vice versa, and hybrids with one monomer in the enzyme dimer from Trypanosoma cruzi, and the second monomer from Trypanosoma brucei. Solvent exposure of the interfacial C15 depends predominantly on the characteristics of the adjoining monomer. Half of the activity of each monomer depends on the integrity of each of the two C15-loop3 portions of the interface | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methylmethane thiosulfonate | modification at C15 in the dimer interface, inducing abolition of catalysis and structural changes. Susceptibility of Trypanosoma cruzi enzyme to modification of C15 is nearly 100fold higher than susceptibility of C15 of Trypanosoma brucei | Trypanosoma brucei | |
methylmethane thiosulfonate | modification at C15 in the dimer interface, inducing abolition of catalysis and structural changes. Susceptibility of Trypanosoma cruzi enzyme to modification of C15 is nearly 100fold higher than susceptibility of C15 of Trypanosoma brucei | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
D-glyceraldehyde 3-phosphate | mutant E168D, pH 7.4, 25°C | Trypanosoma brucei | |
0.28 | - |
D-glyceraldehyde 3-phosphate | in complex with mutant C15A of Trypanosoma cruzi enzyme, pH 7.4, 25°C | Trypanosoma brucei | |
0.28 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, pH 7.4, 25°C | Trypanosoma cruzi | |
0.31 | - |
D-glyceraldehyde 3-phosphate | in complex with monomer from Trypanosoma brucei, pH 7.4, 25°C | Trypanosoma cruzi | |
0.31 | - |
D-glyceraldehyde 3-phosphate | in complex with monomer from Trypanosoma cruzi, pH 7.4, 25°C | Trypanosoma brucei | |
0.42 | - |
D-glyceraldehyde 3-phosphate | mutant C15A, pH 7.4, 25°C | Trypanosoma cruzi | |
0.45 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Trypanosoma cruzi | |
0.46 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Trypanosoma brucei | |
0.62 | - |
D-glyceraldehyde 3-phosphate | in complex with mutant C15A of Trypanosoma cruzi enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C | Trypanosoma brucei | |
0.62 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C | Trypanosoma cruzi | |
0.69 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with mutant E168D of Trypanosoma brucei, pH 7.4, 25°C | Trypanosoma cruzi | |
0.69 | - |
D-glyceraldehyde 3-phosphate | mutant E168D in complex with mutant C15A of Trypanosoma cruzi, pH 7.4, 25°C | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma cruzi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | dihydroxyacetone phosphate | - |
? | |
D-glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | dihydroxyacetone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
D-glyceraldehyde 3-phosphate | mutant E168D, pH 7.4, 25°C | Trypanosoma brucei | |
750 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with mutant E168D of Trypanosoma brucei, pH 7.4, 25°C | Trypanosoma cruzi | |
750 | - |
D-glyceraldehyde 3-phosphate | mutant E168D in complex with mutant C15A of Trypanosoma cruzi, pH 7.4, 25°C | Trypanosoma brucei | |
833 | - |
D-glyceraldehyde 3-phosphate | in complex with mutant C15A of Trypanosoma cruzi enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C | Trypanosoma brucei | |
833 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, after modification by methylmethane thiosulfonate, pH 7.4, 25°C | Trypanosoma cruzi | |
2170 | - |
D-glyceraldehyde 3-phosphate | in complex with mutant C15A of Trypanosoma cruzi enzyme, pH 7.4, 25°C | Trypanosoma brucei | |
2170 | - |
D-glyceraldehyde 3-phosphate | mutant C15A in complex with intact monomer from Trypanosoma brucei enzyme, pH 7.4, 25°C | Trypanosoma cruzi | |
2670 | - |
D-glyceraldehyde 3-phosphate | mutant C15A, pH 7.4, 25°C | Trypanosoma cruzi | |
4500 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Trypanosoma cruzi | |
5000 | - |
D-glyceraldehyde 3-phosphate | in complex with monomer from Trypanosoma brucei, pH 7.4, 25°C | Trypanosoma cruzi | |
5000 | - |
D-glyceraldehyde 3-phosphate | in complex with monomer from Trypanosoma cruzi, pH 7.4, 25°C | Trypanosoma brucei | |
5170 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.4, 25°C | Trypanosoma brucei |