Literature summary for 5.3.1.1 extracted from

Lopez-Velazquez, G.; Molina-Ortiz, D.; Cabrera, N.; Hernandez-Alcantara, G.; Peon-Peralta, J.; Yepez-Mulia, L.; Perez-Montfort, R.; Reyes-Vivas, H.
An unusual triosephosphate isomerase from the early divergent eukaryote Giardia lamblia (2004), Proteins, 55, 824-834.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli TOP10	Giardia intestinalis

Inhibitors

Inhibitors	Comment	Organism	Structure
2-Phosphoglycolate	-	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.53	-	D-glyceraldehyde 3-phosphate	25°C, pH 7.4, dimeric enzyme form	Giardia intestinalis
0.87	-	D-glyceraldehyde 3-phosphate	25°C, pH 7.4, tetrameric enzyme form	Giardia intestinalis
1.1	-	dihydroxyacetone phosphate	25°C, pH 7.4, dimeric enzyme form	Giardia intestinalis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Giardia intestinalis	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	2 * 27000, SDS-PAGE	Giardia intestinalis
27000	-	4 * 27000, SDS-PAGE	Giardia intestinalis
55000	-	dimer, gel filtration	Giardia intestinalis
108000	-	tetramer, gel filtration	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Giardia intestinalis

Source Tissue

Source Tissue	Comment	Organism	Textmining
trophozoite	-	Giardia intestinalis	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3800	-	-	Giardia intestinalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde 3-phosphate	-	Giardia intestinalis	dihydroxyacetone phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 27000, SDS-PAGE	Giardia intestinalis
More	tetramer results from covalent attachment of two dimers that conserve similar association constants between their constituent monomers	Giardia intestinalis
tetramer	4 * 27000, SDS-PAGE	Giardia intestinalis

Synonyms

Synonyms	Comment	Organism
GlTIM	-	Giardia intestinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
267	-	dihydroxyacetone phosphate	25°C, pH 7.4, dimeric enzyme form	Giardia intestinalis
2450	-	D-glyceraldehyde 3-phosphate	25°C, pH 7.4, tetrameric enzyme form	Giardia intestinalis
4833	-	D-glyceraldehyde 3-phosphate	25°C, pH 7.4, dimeric enzyme form	Giardia intestinalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.043	-	2-Phosphoglycolate	25°C, pH 7.4, dimeric enzyme form	Giardia intestinalis

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Release 2023.1 (January 2023)