Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.1 extracted from

  • Gonzalez-Mondragon, E.; Zubillaga, R.A.; Saavedra, E.; Chanez-Cardenas, M.E.; Perez-Montfort, R.; Hernandez-Arana, A.
    Conserved cysteine 126 in triosephosphate isomerase is required not for enzymatic activity but for proper folding and stability (2004), Biochemistry, 43, 3255-3263.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant enzymes C126S and C126A in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C126A turnover number for D-glyceraldehyde 3-phosphate is 1.5fold lower than the wild-type value, KM-value for D-glyceraldehyde 3-phosphate is 1.4fold lower than the wild-type value, turnover number for dihydroxyacetone phosphate is 4.3fold lower than the wild-type value, KM-value for dihydroxyacetone phosphate is 3.7fold lower than the wild-type value Saccharomyces cerevisiae
C126S mutant enzyme shows greater susceptibility to thermal denaturation than wild-type enzyme, turnover number for dihydroxyacetone phosphate is 8.3fold lower than the wild-type value, KM-value for dihydroxyacetone phosphate is 3.5fold lower than the wild-type value Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
D-glyceraldehyde 3-phosphate 25°C, pH 7.4, mutant enzyme C126S Saccharomyces cerevisiae
0.6
-
dihydroxyacetone phosphate 25°C, pH 7.4, mutant enzyme C126S Saccharomyces cerevisiae
0.8
-
D-glyceraldehyde 3-phosphate 25°C, pH 7.4, mutant enzyme C126A Saccharomyces cerevisiae
1.1
-
D-glyceraldehyde 3-phosphate 25°C, pH 7., wild-type enzyme Saccharomyces cerevisiae
2.1
-
dihydroxyacetone phosphate 25°C, pH 7.4, wild-type enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Saccharomyces cerevisiae dihydroxyacetone phosphate
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
dihydroxyacetone phosphate 25°C, pH 7.4, mutant enzyme C126S Saccharomyces cerevisiae
500
-
dihydroxyacetone phosphate 25°C, pH 7.4, wild-type enzyme Saccharomyces cerevisiae
1100
-
D-glyceraldehyde 3-phosphate 25°C, pH 7.4, mutant enzyme C126S Saccharomyces cerevisiae
3100
-
D-glyceraldehyde 3-phosphate 25°C, pH 7.4, mutant enzyme C126A Saccharomyces cerevisiae
4700
-
D-glyceraldehyde 3-phosphate 25°C, pH 7., wild-type enzyme Saccharomyces cerevisiae