Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant enzymes C126S and C126A in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C126A | turnover number for D-glyceraldehyde 3-phosphate is 1.5fold lower than the wild-type value, KM-value for D-glyceraldehyde 3-phosphate is 1.4fold lower than the wild-type value, turnover number for dihydroxyacetone phosphate is 4.3fold lower than the wild-type value, KM-value for dihydroxyacetone phosphate is 3.7fold lower than the wild-type value | Saccharomyces cerevisiae |
C126S | mutant enzyme shows greater susceptibility to thermal denaturation than wild-type enzyme, turnover number for dihydroxyacetone phosphate is 8.3fold lower than the wild-type value, KM-value for dihydroxyacetone phosphate is 3.5fold lower than the wild-type value | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7.4, mutant enzyme C126S | Saccharomyces cerevisiae | |
0.6 | - |
dihydroxyacetone phosphate | 25°C, pH 7.4, mutant enzyme C126S | Saccharomyces cerevisiae | |
0.8 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7.4, mutant enzyme C126A | Saccharomyces cerevisiae | |
1.1 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7., wild-type enzyme | Saccharomyces cerevisiae | |
2.1 | - |
dihydroxyacetone phosphate | 25°C, pH 7.4, wild-type enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | dihydroxyacetone phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
dihydroxyacetone phosphate | 25°C, pH 7.4, mutant enzyme C126S | Saccharomyces cerevisiae | |
500 | - |
dihydroxyacetone phosphate | 25°C, pH 7.4, wild-type enzyme | Saccharomyces cerevisiae | |
1100 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7.4, mutant enzyme C126S | Saccharomyces cerevisiae | |
3100 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7.4, mutant enzyme C126A | Saccharomyces cerevisiae | |
4700 | - |
D-glyceraldehyde 3-phosphate | 25°C, pH 7., wild-type enzyme | Saccharomyces cerevisiae |