Protein Variants | Comment | Organism |
---|---|---|
K174G/T175G/A176G | 16.5fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 7.9fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8fold decrease in turnover number for dihydroxyacetone phosphate, 2.6fold increase in Ki-value for arsenate, 10.4fold increase in Ki-value for 2-phosphoglycolate | Gallus gallus |
V167G/W168G | 17.9fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 2.6fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8.6fold decrease in turnover number for dihydroxyacetone phosphate, 2.7fold increase in Ki-value for arsenate, 8.1fold increase in Ki-value for 2-phosphoglycolate | Gallus gallus |
V167G/W168G/K174G/T175G/A176G | 2529fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 8.5fold increase in Km-value for D-glyceraldehyde 3-phosphate, 1720fold decrease in turnover number for dihydroxyacetone phosphate, 2.8fold increase in Ki-value for arsenate, 12.4fold increase in Ki-value for 2-phosphoglycolate | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phosphoglycolate | - |
Gallus gallus | |
arsenate | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, wild-type enzyme | Gallus gallus | |
0.97 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, wild-type enzyme | Gallus gallus | |
1.2 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G | Gallus gallus | |
3.7 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G | Gallus gallus | |
4 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G | Gallus gallus | |
11 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G | Gallus gallus | |
15 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G | Gallus gallus | |
18 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Gallus gallus | dihydroxyacetone phosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G | Gallus gallus | |
1.7 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G | Gallus gallus | |
50 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G | Gallus gallus | |
54 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G | Gallus gallus | |
240 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme V167G/W168G | Gallus gallus | |
261 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G | Gallus gallus | |
430 | - |
dihydroxyacetone phosphate | 30°C, pH 7.6, wild-type enzyme | Gallus gallus | |
4300 | - |
D-glyceraldehyde 3-phosphate | 30°C, pH 7.6, wild-type enzyme | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
arsenate | 30°C, pH 7.6, wild-type enzyme | Gallus gallus | |
29 | - |
arsenate | 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G | Gallus gallus | |
30 | - |
arsenate | 30°C, pH 7.6, mutant enzyme V167G/W168G | Gallus gallus | |
31 | - |
arsenate | 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G | Gallus gallus |