Literature summary for 5.3.1.1 extracted from

Alvarez, M.; Wouters, J.; Maes, D.; Mainfroid, V.; Rentier-Delrue, F.; Wyns, L.; Depiereux, E.; Martial, J.A.
Lys13 plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures (1999), J. Biol. Chem., 274, 19181-19187.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Geobacillus stearothermophilus
-	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Geobacillus stearothermophilus
hanging drop vapor diffusion method	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
H12N	mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 64°C compared to 68°C for the wild-type enzyme	Geobacillus stearothermophilus
H12N/K13G	mutant enzyme shows increases thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 96°C compared to 94°C of the wild-type enzyme	Thermotoga maritima
H12N/K13G	mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 37°C compared to 68°C for the wild-type enzyme	Geobacillus stearothermophilus
H13G	mutant enzyme with decreased thermal stability compared to wild-type enzyme. Half-life of 11.5 min at 51°C compared to 68°C for the wild-type enzyme	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and mutant enzyme H12N/K13G	Thermotoga maritima
wild-type and mutant enzymes H12N, H13G and H12N/K13G	Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	Lys13 plays a crucial role in the functional adaptation of the thermophilic enzyme to high temperatures	Geobacillus stearothermophilus
37	-	half-life of mutant enzyme H12N/K13G is 11.5 min	Geobacillus stearothermophilus
51	-	half-life of mutant enzyme H13G is 11.5 min	Geobacillus stearothermophilus
64	-	half-life of mutant enzyme H12N is 11.5 min	Geobacillus stearothermophilus
68	-	half-life of wild-type recombinant enzyme is 11.5 min	Geobacillus stearothermophilus
94	-	half-life of wild-type recombinant enzyme is 11.5 min	Thermotoga maritima
96	-	half-life of mutant enzyme H12N/K13G is 11.5 min	Thermotoga maritima

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Release 2023.1 (January 2023)