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Literature summary for 5.3.1.1 extracted from

  • Singh, S.K.; Maithal, K.; Balaram, H.; Balaram, P.
    Synthetic peptides as inactivators of multimeric enzymes: inhibition of Plasmodium falciparum triosephosphate isomerase by interface peptides (2001), FEBS Lett., 501, 19-23.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
Y74C inhibitory effect of KFGNGSYTGEVS and KYGNGSCTGEVS is more pronounced compared to the wild-type enzym. Mutant enzyme has reduced stability due to an interface cavity Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
KFGNGSYTGEVS peptide that corresponds to loop 3 of the triosephosphate isomerase, residues 68-79. Efficient inhibitor with the activity falling to about 45% at 1000fold molar excess of the peptide in case of the wild-type enzyme. In the case of either of the mutants, Y74C and Y74G, even at 100fold molar excess of the peptide, only 30% activity can be obtained Plasmodium falciparum
KYGNGSCTGEVS peptide that is an analog of the peptide that corresponds to loop 3 of the protein, residues 68-79, with the replacement Y74C and F69Y. Inhibits the activity of the mutant enzymes Y74C and Y74G, with about 40% activity remaining in the presence of 1000fold molar excess of the peptide Plasmodium falciparum
additional information ANWKCNGTLE, the peptide that corresponds to loop 1 of triosephosphate isomerase, residues 9-18, shows only negligible inhibition of wild-type enzyme and mutant enzymes Y74G and Y74C, with a fall in the enzymatic activity by only about 20% at 1000fold molar excess of the peptide Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
D-glyceraldehyde 3-phosphate pH 7.6, mutant enzyme Y74C Plasmodium falciparum
0.34
-
D-glyceraldehyde 3-phosphate pH 7.6, mutant enzyme Y74G Plasmodium falciparum
0.35
-
D-glyceraldehyde 3-phosphate pH 7.6, wild-type enzyme Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q07412
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and mutant enzymes Y74C and Y74G Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity of wild-type and mutant enzymes Y74C and Y74G Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate
-
Plasmodium falciparum Glycerone phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
67
-
D-glyceraldehyde 3-phosphate pH 7.6, mutant enzyme Y74G Plasmodium falciparum
2417
-
D-glyceraldehyde 3-phosphate pH 7.6, mutant enzyme Y74C Plasmodium falciparum
2420
-
D-glyceraldehyde 3-phosphate pH 7.6, mutant enzyme Y74C Plasmodium falciparum
4467
-
D-glyceraldehyde 3-phosphate pH 7.6, wild-type enzyme Plasmodium falciparum
4470
-
D-glyceraldehyde 3-phosphate pH 7.6, wild-type enzyme Plasmodium falciparum