Protein Variants | Comment | Organism |
---|---|---|
Y74C | inhibitory effect of KFGNGSYTGEVS and KYGNGSCTGEVS is more pronounced compared to the wild-type enzym. Mutant enzyme has reduced stability due to an interface cavity | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KFGNGSYTGEVS | peptide that corresponds to loop 3 of the triosephosphate isomerase, residues 68-79. Efficient inhibitor with the activity falling to about 45% at 1000fold molar excess of the peptide in case of the wild-type enzyme. In the case of either of the mutants, Y74C and Y74G, even at 100fold molar excess of the peptide, only 30% activity can be obtained | Plasmodium falciparum | |
KYGNGSCTGEVS | peptide that is an analog of the peptide that corresponds to loop 3 of the protein, residues 68-79, with the replacement Y74C and F69Y. Inhibits the activity of the mutant enzymes Y74C and Y74G, with about 40% activity remaining in the presence of 1000fold molar excess of the peptide | Plasmodium falciparum | |
additional information | ANWKCNGTLE, the peptide that corresponds to loop 1 of triosephosphate isomerase, residues 9-18, shows only negligible inhibition of wild-type enzyme and mutant enzymes Y74G and Y74C, with a fall in the enzymatic activity by only about 20% at 1000fold molar excess of the peptide | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme Y74C | Plasmodium falciparum | |
0.34 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme Y74G | Plasmodium falciparum | |
0.35 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q07412 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and mutant enzymes Y74C and Y74G | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of wild-type and mutant enzymes Y74C and Y74G | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Plasmodium falciparum | Glycerone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
67 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme Y74G | Plasmodium falciparum | |
2417 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme Y74C | Plasmodium falciparum | |
2420 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, mutant enzyme Y74C | Plasmodium falciparum | |
4467 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Plasmodium falciparum | |
4470 | - |
D-glyceraldehyde 3-phosphate | pH 7.6, wild-type enzyme | Plasmodium falciparum |