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Literature summary for 5.3.1.1 extracted from
- Unfolding of Plasmodium falciparum triosephosphate isomerase in urea and guanidinium chloride: evidence for a novel disulfide exchange reaction in a covalently cross-linked mutant (1999), Biochemistry, 38, 423-431.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Y74C | urea unfolding profiles of Y74Cox in urea solution obtained by fluorescence and circular dichroism approximate a two-state transition and do not show the presence of stable intermediates over a wide range of denaturant concentrations. In wild-type enzyme the unfolding results in gradual change in spectroscopic properties. The unfolding transition midpoit is 3.5 M for Y74Cox and 5.5 M for the wild-type enzyme | Plasmodium falciparum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Plasmodium falciparum |
| 27832 | - |
x * 27832, electrospray mass spectrometry | Plasmodium falciparum |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | 2.4 M, the unfolding transition is complete | Plasmodium falciparum |
| urea | the unfolding transition midpoit is 3.5 M for Y74Cox and 5.5 M for the wild-type enzyme. The dimeric wild-type enzyme retains considerable secondary, tertiary, and quarternary structure even in 8 M urea. Urea unfolding profiles of Y74Cox in urea solution obtained by fluorescence and circular dichroism approximate a two-state transition and do not show the presence of stable intermediates over a wide range of denaturant concentrations. In wild-type enzyme the unfolding results in gradual change in spectroscopic properties | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme Y74C | Plasmodium falciparum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Plasmodium falciparum |
| ? | x * 27832, electrospray mass spectrometry | Plasmodium falciparum |
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