Protein Variants | Comment | Organism |
---|---|---|
C113D | mutation does not compromise isoform Pin1 function in vivo nor does it abolish catalytic activity. C113 may not be the catalytic nucleophile | Homo sapiens |
C113S | decrease in kcat, small in Km value. C113 may not be the catalytic nucleophile | Homo sapiens |
K63A | decrease in kcat, increase in Km value | Homo sapiens |
additional information | analysis of residues conserved in natural Pin1 sequences and during unigenic evolution and screen for completely conserved residues in functional Pin1 mutants by complementation of Ess1 mutant in Saccharomyces cerevisiae | Homo sapiens |
R68A/R69A | decrease in both kcat and Km value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant R68A/R69A, 0°C, pH 7.5 | Homo sapiens | |
0.31 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | wild-type, 0°C, pH 7.5 | Homo sapiens | |
0.37 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant C113D, 0°C, pH 7.5 | Homo sapiens | |
0.41 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant C113S, 0°C, pH 7.5 | Homo sapiens | |
0.7 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant K63A, 0°C, pH 7.5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13526 | isoform Pin1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | - |
Homo sapiens | N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant C113S, 0°C, pH 7.5 | Homo sapiens | |
11 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant K63A, 0°C, pH 7.5 | Homo sapiens | |
18 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant R68A/R69A, 0°C, pH 7.5 | Homo sapiens | |
29 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | mutant C113D, 0°C, pH 7.5 | Homo sapiens | |
140 | - |
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | wild-type, 0°C, pH 7.5 | Homo sapiens |