Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.2.1.8 extracted from

  • Behrsin, C.D.; Bailey, M.L.; Bateman, K.S.; Hamilton, K.S.; Wahl, L.M.; Brandl, C.J.; Shilton, B.H.; Litchfield, D.W.
    Functionally important residues in the peptidyl-prolyl isomerase Pin1 revealed by unigenic evolution (2007), J. Mol. Biol., 365, 1143-1162.
    View publication on PubMed
Search for more literature for 5.2.1.8

Protein Variants

Protein Variants Comment Organism
C113D mutation does not compromise isoform Pin1 function in vivo nor does it abolish catalytic activity. C113 may not be the catalytic nucleophile Homo sapiens
C113S decrease in kcat, small in Km value. C113 may not be the catalytic nucleophile Homo sapiens
K63A decrease in kcat, increase in Km value Homo sapiens
additional information analysis of residues conserved in natural Pin1 sequences and during unigenic evolution and screen for completely conserved residues in functional Pin1 mutants by complementation of Ess1 mutant in Saccharomyces cerevisiae Homo sapiens
R68A/R69A decrease in both kcat and Km value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant R68A/R69A, 0°C, pH 7.5 Homo sapiens
0.31
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide wild-type, 0°C, pH 7.5 Homo sapiens
0.37
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant C113D, 0°C, pH 7.5 Homo sapiens
0.41
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant C113S, 0°C, pH 7.5 Homo sapiens
0.7
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant K63A, 0°C, pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13526 isoform Pin1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide
-
 Homo sapiens N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant C113S, 0°C, pH 7.5 Homo sapiens
11
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant K63A, 0°C, pH 7.5 Homo sapiens
18
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant R68A/R69A, 0°C, pH 7.5 Homo sapiens
29
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide mutant C113D, 0°C, pH 7.5 Homo sapiens
140
-
 N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide wild-type, 0°C, pH 7.5 Homo sapiens