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Literature summary for 5.2.1.4 extracted from
- The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in Corynebacterium glutamicum (2006), J. Biol. Chem., 281, 10778-10785.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | weak activator at 1 mM | Corynebacterium glutamicum |  |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | mycothiol as target for clinical treatment | Corynebacterium glutamicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibitory at 10 mM | Corynebacterium glutamicum | |
| Fe3+ | weak inhibitor | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 148.4 | - |
3-maleylpyruvate | - |
Corynebacterium glutamicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Mg2+, Ca2+, Cu2+, Ni2+, Mn2+ have no effect | Corynebacterium glutamicum | |
| Zn2+ | activating | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
SDS-PAGE | Corynebacterium glutamicum |
| 34000 | - |
gel filtration | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | Q8NLC1 | wild type; mshA-, mshB-, mshC-, mshD-deletion mutants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant enzyme | Corynebacterium glutamicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| at -80°C | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-maleylpyruvate + | - |
Corynebacterium glutamicum | 3-fumarylpyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 x 33000, SDS-Page | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-maleylpyruvate cis-trans-isomerase | - |
Corynebacterium glutamicum |
| maleylpyruvate isomerase | - |
Corynebacterium glutamicum |
| MDMPI | mycothiol dependent maleylpyruvate isomerase | Corynebacterium glutamicum |
| msh gene | - |
Corynebacterium glutamicum |
| ncgl2918 gene | - |
Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | MSH, mycothiol, 1D-myo-inosityl-1-(N-acetyl-L-cysteinyl)amido-2-deoxy-a-D-glucopyranoside, purified and identified by HPLC and mass spectrometric analysis | Corynebacterium glutamicum |
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